PMID: 6164387Mar 17, 1981Paper

Synthesis of mono- and dinucleotide photoaffinity probes of ribonucleic acid polymerase

Biochemistry
L H DeRiemer, C F Meares

Abstract

The abortive initiation reaction of RNA polymerase has been used to prepare adenylyl-(3'--5')-uridine 5'-phosphate (pApU) in 74% yield from AMP and UTP. The reactive intermediate p-azidophenyl phosphorimidazolidate has been prepared by starting from p-nitrophenyl phosphate. Reaction of this compound with the terminal phosphates of adenosine 5'-phosphate and adenylyl-(3'--5')-uridine 5'-phosphate gives the corresponding beta-substituted 5'-diphosphates. These products are incorporated into the 5' (leading) end of RNA by RNA polymerase (Escherichia coli) and can be photoactivated at a specific stage of RNA elongation. The dinucleotide photoaffinity label beta-(4-azidophenyl) adenylyl-(3'--5')-uridine 5'-diphosphate stimulates RNA synthesis more strongly than adenylyl-(3'--5')-uridine.

References

May 1, 1977·Analytical Biochemistry·J J Sedmak, S E Grossberg
Apr 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·H Oen, C W Wu
Nov 1, 1976·European Journal of Biochemistry·V W Armstrong, F Eckstein
Jan 1, 1977·Methods in Enzymology·H Bayley, J R Knowles
Apr 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·J A Maassen, W Möller
Apr 20, 1965·Journal of the American Chemical Society·D E HOARD, D G OTT

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Citations

Jan 1, 1991·Journal of Pharmaceutical and Biomedical Analysis·J N MillerM T French
Jan 15, 1997·Journal of Immunological Methods·K KronkvistG Johansson
Oct 31, 2008·Talanta·M PravdaG G Guilbault
Dec 19, 2001·Journal of Photochemistry and Photobiology. B, Biology·P V AlekseyevT S Godovikova
Jun 19, 2002·Analytical Chemistry·Tzyh-Chyang TangHsuan-Jung Huang

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