PMID: 6159635Aug 1, 1980Paper

Synthesis of new proteins associated with the induction of interferon in human fibroblast cells

Proceedings of the National Academy of Sciences of the United States of America
N B Raj, P M Pitha

Abstract

The relative amounts of translatable cellular mRNAs and newly synthesized cellular proteins were examined in poly(I) x poly(C)-induced human fibroblast cells early during induction. At this time interferon and interferon mRNA synthesis are maximal and cells have not acquired their antiviral thesis are maximal and cells have not acquired their antiviral state. Translation of the mRNA from poly(I) x poly(C)-induced cells in a wheat germ cell-free system led to the synthesis of a [35S]methionine-labeled 22,000-dalton protein that is precipitated by antiserum to highly purified human fibroblast interferon. The synthesis of this protein was detected only with the mRNA preparations that, when translated in Xenopus oocytes, coded for the synthesis of biologically active human interferon. Two-dimensional gel analysis of the [35S]methionine-labeled polypeptides translated from the total mRNA of the induced and uninduced cells revealed the presence of 23 new proteins that were translated from mRNAs of the induced cells but not from the mRNAs of the controls. These polypeptides ranged from 15,000 to 70,000 daltons. Thirteen of these proteins were detected in induced cells labeled with [35S]methionine. It is concluded that, in human fibrob...Continue Reading

References

Apr 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·N B Raj, P M Pitha
Aug 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·P B SehgalI Tamm
Oct 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·R L CavalieriS Pestka
Jan 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·I M Kerr, R E Brown
Feb 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·M RubinsteinS Pestka
Apr 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·E Knight, B D Korant
Aug 1, 1976·European Journal of Biochemistry·H R Pelham, R J Jackson
Dec 11, 1975·Proceedings of the National Academy of Sciences of the United States of America·F H ReynoldsP M Pitha
Feb 11, 1976·Proceedings of the National Academy of Sciences of the United States of America·E Knight
May 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·P B Sehgal, I Tamm
Sep 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·B LebleuP Lengyel
Jul 18, 1975·Biochemical and Biophysical Research Communications·F H Reynolds, P M Pitha
Jan 1, 1964·Biochemical and Biophysical Research Communications·J Taylor
Aug 5, 1974·Biochemical and Biophysical Research Communications·F H Reynolds, P M Pitha
Aug 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·B E Roberts, B M Paterson
May 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·J de Maeyer-GuignardL Montagnier
Jul 1, 1974·European Journal of Biochemistry·W M Bonner, R A Laskey
Sep 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·Y H TanM Ho

❮ Previous
Next ❯

Citations

Dec 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·N B Raj, P M Pitha
Jul 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·P M PithaG S Hayward
Sep 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·D Canaani, P Berg
Feb 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·S ChatterjeeE Hunter
May 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·J ContentW Fiers
Jul 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·N B Raj, P M Pitha
Jan 1, 1981·Journal of Interferon Research·N B Raj, P M Pitha
Jan 1, 1985·Journal of Interferon Research·N B RajP M Pitha
Feb 1, 1990·Journal of Interferon Research·H Y LinH R Thacore
Sep 15, 1986·European Journal of Biochemistry·G HaegemanW Fiers
Jun 1, 1988·European Journal of Biochemistry·M G WatheletG A Huez
Feb 1, 2009·Proteomics. Clinical Applications·Deborah Penque
Jan 1, 1992·Experimental Cell Research·M A PerriconeD M Hyde
Jan 1, 1982·Journal of Interferon Research·A R NeurathP M Pitha

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antivirals

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Antivirals (ASM)

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.