PMID: 7459333Nov 25, 1980Paper

Synthesis of peptide spin-labels that bind to neurophysin and their application to distance measurements within neurophysin complexes

Biochemistry
S T Lord, E Breslow

Abstract

The synthesis of two spin-labels capable of binding to the hormone-binding site(s) of neurophysin is described. The two spin-labels are 4-(glycyl-L-phenylalanylamido)-2,2,6,6-tetramethylpiperidinyl-1-oxy and S-[[[3-(2,2,5,5-tetramethylpyrrolidine-1-oxy)amino]carbonyl]methyl]-L-cysteinyl -L-tyrosine amide; synthesis of the former is achieved by a novel route to circumvent problems associated with nitroxide instability under standard conditions of peptide deblocking. NMR studies of the effects of binding these spin-labels on relaxation rates of individual proton resonances of neurophysin were used to calculate correlation times and distances between the bound nitroxides and the observed protons. The results indicate that residue 3 of peptides bound to the strong site of neurophysin is greater than or equal to 14 A from Tyr-49 and argue against a distance of < 5 A between the ortho ring protons of Tyr-49 and those of residue 2 of peptides bound to the strong site. Alternatively, the data suggest that the previously observed nuclear Overhauser effect between these protons reflects spin diffusion at the strong site and a contribution of uncertain magnitude from a second but very weak binding site; this second site is close to Tyr-49...Continue Reading

References

Feb 21, 1975·Annals of the New York Academy of Sciences·V J HrubyJ F McKelvy
Feb 21, 1975·Annals of the New York Academy of Sciences·P CohenJ H. Griffin
Jan 1, 1979·Annual Review of Biochemistry·E Breslow
Jan 13, 1978·Biochemical and Biophysical Research Communications·S T Lord, E Breslow
Feb 21, 1975·Annals of the New York Academy of Sciences·I M ChaikenH C Taylor
Feb 1, 1970·The Biochemical Journal·A J Furth, D B Hope

Citations

Jan 19, 2011·Progress in Nuclear Magnetic Resonance Spectroscopy·Peter H J Keizers, Marcellus Ubbink
Nov 1, 1991·International Journal of Peptide and Protein Research·C A HasselbacherW R Laws
Feb 1, 1983·International Journal of Peptide and Protein Research·V Virmani-Sardana, E Breslow

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