Dec 5, 2014

Extended depth of focus multiphoton microscopy via incoherent pulse splitting

BioRxiv : the Preprint Server for Biology
Cody HinchliffKaren Ann Cranston


We present a phase mask that can be easily added to any multi-photon raster scanning microscope to extend the depth of focus five-fold at a small loss in lateral resolution. The method is designed for ultrafast laser pulses or other light-sources featuring a low coherence length. In contrast to other methods of focus extension, our approach uniquely combines low complexity, high light-throughput and multicolor capability. We characterize the point-spread function in a two-photon microscope and demonstrate fluorescence imaging of GFP labeled neurons in fixed brain samples as imaged with conventional and extended depth of focus two-photon microscopy.

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Mentioned in this Paper

Trees (plant)
Phylogenetic Analysis
Comparative Biology
Homologous Gene
Molecular Assembly/Self Assembly

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