PMID: 9418250Jan 7, 1998Paper

Synthesis of poly(3-hydroxyalkanoates) in Escherichia coli expressing the PHA synthase gene phaC2 from Pseudomonas aeruginosa: comparison of PhaC1 and PhaC2

FEMS Microbiology Letters
Qingsheng QiA Steinbüchel

Abstract

In order to obtain functional expression of PHA synthase gene phaC2 from Pseudomonas aeruginosa in Escherichia coli, the coding region of phaC2 was subcloned, including the ribosomal binding site, into pBluescript SK- collinear to the lac promoter. This plasmid pBHR71-C2 enabled functional expression of phaC2 in E. coli LS1298 (fadB) under lac promoter control, leading to PHA accumulation, when grown in LB medium containing 0.5% (w/v) of various fatty acids (C8-C14). The strongest accumulation of PHA was observed, when dodecanoate was provided as carbon source, and PHA contributed to 15% of cell dry weight, which was composed of 35 mol% 3-hydroxydodecanoate, 60 mol% 3-hydroxydecanoate and 5 mol% 3-hydroxyoctanoate. Plasmid pBHR78, which contained both genes phaC1 and phaC2 from P. aeruginosa under lac promoter control in pBluescript SK- led in E. coli LS1298 to PHA accumulation, which contributed to 13% of cell dry weight, when cells were grown on decanoate. Only slight differences in PHA composition compared with either PhaC1 or PhaC2 were obtained. The weight average molecular masses of PHA purified from decanoate-grown cells of E. coli LS1298 expressing PhaC1 or PhaC2 alone or both PHA synthases, were 106 x 10(3), 70 x 10(3)...Continue Reading

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Citations

Mar 12, 2013·Applied Microbiology and Biotechnology·Qian WangQingsheng Qi
Feb 24, 2005·Journal of Industrial Microbiology & Biotechnology·Daniel K Y SolaimanSebastiana Greco
Jul 23, 1999·International Journal of Biological Macromolecules·B H Rehm, A Steinbüchel
Jun 11, 1999·Current Opinion in Biotechnology·B Witholt, B Kessler
Apr 12, 2006·Applied Microbiology and Biotechnology·Enrico ConteAntonino Catara
Apr 1, 2000·Applied and Environmental Microbiology·Q RenB Witholt
Feb 2, 2000·Applied and Environmental Microbiology·S J Liu, A Steinbüchel
Oct 27, 2001·Applied and Environmental Microbiology·M J PettinariB S Méndez
Dec 23, 2015·Journal of Biotechnology·Jie GaoBruce A Ramsay
Nov 14, 2006·Bioresource Technology·Rui LiQingsheng Qi
Jul 15, 2015·Journal of Industrial Microbiology & Biotechnology·Cuijuan GaoCarol Sze Ki Lin
Jan 11, 2014·Biotechnology for Biofuels·Helge Jans Janßen, Alexander Steinbüchel

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