Synthesis of polymer-functionalized nanoscale graphene oxide with different surface charge and its cellular uptake, biosafety and immune responses in Raw264.7 macrophages

Materials Science & Engineering. C, Materials for Biological Applications
Bing WangZhiwen Hu

Abstract

Polymer-functionalized graphene oxide (GO) has superior properties such as large surface area, extraordinary mechanical strength, high carrier mobility, good stability in physiological media and low cytotoxicity, making it an attractive material for drug and gene delivery. Herein, we successfully synthesized GO with an average size of 168.3 nm by a modified Hummers' method. Branched polyethylenimine (PEI) and 6-armed polyethylene glycol (PEG) functionalized GO complexes (GO-PEI and GO-PEG) with different zeta potentials of 47.2 mV and -43.0 mV, respectively, were successfully synthesized through amide linkages between the COOH groups of GO and the NH2 groups of PEI and PEG. Then, the interactions between GO-PEI and GO-PEG complexes and Raw264.7 mouse monocyte-macrophage cells were investigated. The GO-PEI and GO-PEG complexes could both be internalized by Raw264.7 cells. However, compared with the GO-PEG complex, the GO-PEI complex showed higher intracellular delivery efficiency in Raw264.7 cells. Moreover, it was found that the GO-PEI complex not only gathered in endosomes but also in the cytoplasm, whereas GO-PEG gathered in endosomes only. The MTT tests showed that both GO-PEI and GO-PEG complexes exhibited very low cytotoxi...Continue Reading

Citations

Feb 6, 2020·Nanomaterials·Sowmya MalanagahalliAlberto Bianco
Sep 24, 2019·Future Medicinal Chemistry·Raul OrtizJose Prados
Dec 1, 2020·Colloids and Surfaces. B, Biointerfaces·Huan YangYongjun Chen
Feb 9, 2021·Bioactive Materials·Keshav Narayan AlagarsamySanjiv Dhingra
Mar 18, 2021·ACS Biomaterials Science & Engineering·Jamie J GrantAilish Breen
Mar 25, 2021·Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes·Hazel LinAlberto Bianco
Jun 5, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Meili ShenYapeng Li
Jul 3, 2021·International Journal of Molecular Sciences·Mónica CicuéndezMaría Teresa Portolés

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