Synthesis of polypyrrole within the cell wall of yeast by redox-cycling of [Fe(CN)6](3-)/[Fe(CN)6](4-)

Enzyme and Microbial Technology
A RamanaviciusA Ramanaviciene

Abstract

Yeast cells are often used as a model system in various experiments. Moreover, due to their high metabolic activity, yeast cells have a potential to be applied as elements in the design of biofuel cells and biosensors. However a wider application of yeast cells in electrochemical systems is limited due to high electric resistance of their cell wall. In order to reduce this problem we have polymerized conducting polymer polypyrrole (Ppy) directly in the cell wall and/or within periplasmic membrane. In this research the formation of Ppy was induced by [Fe(CN)6](3-)ions, which were generated from K4[Fe(CN)6], which was initially added to polymerization solution. The redox process was catalyzed by oxido-reductases, which are present in the plasma membrane of yeast cells. The formation of Ppy was confirmed by spectrophotometry and atomic force microscopy. It was confirmed that the conducting polymer polypyrrole was formed within periplasmic space and/or within the cell wall of yeast cells, which were incubated in solution containing pyrrole, glucose and [Fe(CN)6](4-). After 24h drying at room temperature we have observed that Ppy-modified yeast cell walls retained their initial spherical form. In contrast to Ppy-modified cells, the ...Continue Reading

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Citations

Jun 18, 2016·Carbohydrate Polymers·Irem Cemre TuruOzlem Yesil-Celiktas
Jan 9, 2018·Critical Reviews in Analytical Chemistry·Megha A DeshmukhArunas Ramanavicius
Dec 21, 2018·Chembiochem : a European Journal of Chemical Biology·Harry G ShermanFrankie J Rawson
Jan 3, 2020·Angewandte Chemie·Mechelle R BennettFrankie J Rawson
Nov 5, 2019·Frontiers in Bioengineering and Biotechnology·Euijin ShimHye Rim Kim
Jan 16, 2021·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Di ChangFumihito Arai
Dec 9, 2020·International Journal of Molecular Sciences·Simonas Ramanavicius, Arunas Ramanavicius
Dec 31, 2020·Polymers·Simonas Ramanavicius, Arunas Ramanavicius
Apr 4, 2021·Polymers·Simonas RamanaviciusArunas Ramanavicius
May 1, 2021·Sensors·Eivydas AndriukonisArunas Ramanavicius
Aug 20, 2019·Analytical Chemistry·Maki SaitoHiroshi Shiigi
Oct 30, 2021·Chemical Reviews·Samantha R McCuskeyThuc-Quyen Nguyen

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