Synthesis of polysaccharide-block-polypeptide copolymer for potential co-delivery of drug and plasmid DNA

Macromolecular Bioscience
Qianqian LiChao Zhang

Abstract

A pH-sensitive, biodegradable, and biocompatible polysaccharide-block-polypeptide Copolymer derivative {Ac-Dex-b-PAsp(DET)} is synthetized from acetal-modified dextran (Ac-Dex) and diethylenetriamine (DET) grafted poly(L-aspartic acid) {PAsp(DET)} by using click and aminolysis reaction. The copolymer can self-assemble into cationic nanopaticles for potential co-delivery of plasmid DNA (pEGFP-N3) and anticancer drug (doxorubicin, DOX), by using water/oil/water (w/o/w) emulsion method. Gel retardation assay reveals that pDNA can be effectively complexed into cationic nanoparticles at N/P ratio = 12. In vitro drug release behavior of DOX-NPs and DOX/pDNA-NPs is achieved by using fluorescence spectra and UV-Vis spectra and confocal laser scanning microscopy (CLSM). And, pEGFP-N3-NPs at N/P ratio = 42 presents the considerable potential in cell transfection. Cell viability assay shows that nanoparticles exhibit low cell cytotoxicity. These results suggest that the copolymer has excellent performance and potential for the co-delivery of gene and drugs.

References

Jan 7, 2003·Nature Medicine·Thomas J Wickham
Mar 15, 2005·Journal of Controlled Release : Official Journal of the Controlled Release Society·Eun Seong LeeYou Han Bae
Sep 11, 2007·Journal of Controlled Release : Official Journal of the Controlled Release Society·Eun Seong LeeYou Han Bae
Jan 12, 2008·Journal of Controlled Release : Official Journal of the Controlled Release Society·Haiqing YinYou Han Bae
Jul 18, 2008·Journal of the American Chemical Society·Eric M BachelderJean M J Fréchet
Mar 27, 2009·Proceedings of the National Academy of Sciences of the United States of America·Kyle E BroadersJean M J Fréchet
Jul 10, 2010·Nature Reviews. Drug Discovery·Robby A Petros, Joseph M DeSimone
Jul 2, 2011·Nature Reviews. Drug Discovery·Jin-Wook YooSamir Mitragotri
Apr 10, 2012·Biomaterials·Shuhuan LiuChen Jiang
Nov 28, 2012·Advanced Healthcare Materials·Majad KhanYi-Yan Yang
Sep 26, 2013·Advanced Drug Delivery Reviews·Yuanpei LiKit S Lam
Oct 24, 2013·Nature Materials·Simona MuraPatrick Couvreur

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Citations

Aug 20, 2015·Macromolecular Bioscience·Ping WangHua Yang
Oct 8, 2016·Nanoscale·Kegang LiuPatrick Hunziker
Oct 5, 2018·Scientific Reports·Haohao DuanSebastien Lecommandoux
Nov 14, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Wen LiuWei Xue
Apr 3, 2019·Biomacromolecules·Emily T Graham, Kyle E Broaders
Jul 27, 2017·Biomacromolecules·Benjamin B BreitenbachPeter R Wich

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