PMID: 1212202Sep 1, 1975Paper

Synthesis of proline and hydroxyproline in human lung (WI-38) fibroblasts

The Biochemical Journal
T F Shen, H J Strecker

Abstract

Human lung fibroblasts (WI-38) in late exponential phase of growth, in stationary phase after confluency was reached, and at high or low number of population doublings were used to investigate the synthesis of proline and hydroxyproline from glutamate or arginine. Glutamate was from two to five times as effective a precursor as arginine; glutamine did not seem to be involved in these metabolic pathways. Accumulation of protein-bound hydroxyproline in cell layers was observed only after confluency. Confluent cells synthesized more proline from glutamate than did cells in late exponential growth. Conversion of glutamate into intracellular free proline was conducted also to a greater extent in confluent cells at a high number of population doublings. Conversion of glutamate into proline or hydroxyproline in cell-layer protein was not affected significantly by the number of population doublings. Less total protein as well as less hydroxyproline accumulated with cells at a high number of population doublings.

Citations

Mar 1, 1979·Journal of Cellular Physiology·R J Smith, J M Phang
Oct 24, 2007·Applied Microbiology and Biotechnology·Haiyan HuangYuanxing Zhang
Apr 11, 1988·The American Journal of Physiology·J E AlbinaM D Caldwell
Sep 21, 1995·Biochimica Et Biophysica Acta·G BellonJ P Borel
May 1, 1978·Life Sciences·J J Wasmuth, C T Caskey
Jan 1, 1983·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·F J HirdR E Niekrash
Oct 15, 1983·Archives of Biochemistry and Biophysics·J G HensleeM E Jones

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