PMID: 333448Sep 1, 1977Paper

Synthesis of prostaglandin I2 (prostacyclin) by cultured human and bovine endothelial cells

Proceedings of the National Academy of Sciences of the United States of America
B B WekslerE A Jaffe


Cultured endothelial cells derived from human umbilical veins or bovine aorta produce a potent inhibitor of platelet aggregation. The inhibitor is synthesized from sodium arachidonate or or prostaglandin endoperoxides by a microsomal enzyme system. Tranylcypromine, a specific antagonist of prostacyclin synthetase, suppresses production of the inhibitor by endothelial cells. The inhibitor, which is ether extractable, has been identified using a two-step thin-layer radiochromatographic procedure and a synthetic prostaglandin I2 standard. With this procedure, we have shown that human and bovine endothelial cells convert sodium [3H]arachidonate to radiolabeled prostaglandin I2 and 6-keto-prostaglandin F1alpha, as wellas prostaglandin E2. Thus, endothelial cells may be non-thrombogenic in vivo because they synthesize and release prostaglandin I2, a potent inhibitor of platelet aggregation.

Associated Clinical Trials


Dec 1, 1976·Prostaglandins·N WhittakerF F Sun
Mar 1, 1977·Prostaglandins·R R GormanO V Miller
Aug 19, 1976·The New England Journal of Medicine·R Ross, J A Glomset
Jan 24, 1977·Biochemical and Biophysical Research Communications·P P HoC P Walters
Mar 16, 1977·Journal of the American Chemical Society·E J CoreyI Szekely
Sep 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·E J CoreyB Samuelsson

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