PMID: 333448Sep 1, 1977Paper

Synthesis of prostaglandin I2 (prostacyclin) by cultured human and bovine endothelial cells

Proceedings of the National Academy of Sciences of the United States of America
B B WekslerE A Jaffe

Abstract

Cultured endothelial cells derived from human umbilical veins or bovine aorta produce a potent inhibitor of platelet aggregation. The inhibitor is synthesized from sodium arachidonate or or prostaglandin endoperoxides by a microsomal enzyme system. Tranylcypromine, a specific antagonist of prostacyclin synthetase, suppresses production of the inhibitor by endothelial cells. The inhibitor, which is ether extractable, has been identified using a two-step thin-layer radiochromatographic procedure and a synthetic prostaglandin I2 standard. With this procedure, we have shown that human and bovine endothelial cells convert sodium [3H]arachidonate to radiolabeled prostaglandin I2 and 6-keto-prostaglandin F1alpha, as wellas prostaglandin E2. Thus, endothelial cells may be non-thrombogenic in vivo because they synthesize and release prostaglandin I2, a potent inhibitor of platelet aggregation.

Associated Clinical Trials

References

Dec 1, 1976·Prostaglandins·N WhittakerF F Sun
Mar 1, 1977·Prostaglandins·R R GormanO V Miller
Aug 19, 1976·The New England Journal of Medicine·R Ross, J A Glomset
Jan 24, 1977·Biochemical and Biophysical Research Communications·P P HoC P Walters
Mar 16, 1977·Journal of the American Chemical Society·E J CoreyI Szekely
Sep 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·E J CoreyB Samuelsson

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Citations

Jul 1, 1994·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·F SunL A Fitzpatrick
Jan 1, 1991·Journal of Clinical Laboratory Analysis·S K PengR J Morin
Jan 1, 1992·Cytotechnology·S P Massia, J A Hubbell
Dec 22, 1978·Molecular and Cellular Biochemistry·M I Barnhart
Sep 1, 1985·Basic Research in Cardiology·K Schrör
Dec 1, 1981·Agents and Actions·E McCallD C Dumonde
Aug 1, 1987·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·O Tokunaga, T Watanabe
Mar 1, 1985·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·I E GoetzD N Krause
Mar 1, 1988·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·T A McCaffreyB B Weksler
Jan 9, 1985·Biochimica Et Biophysica Acta·M Hee-CheongD L Severson
Jun 1, 1979·European Journal of Pharmacology·M L OgletreeK C Nicolaou
May 21, 1982·European Journal of Pharmacology·J Talesnik, J C Hsia
Nov 27, 1984·European Journal of Pharmacology·P Ioannou, J Talesnik
Jan 1, 1989·Experimental Cell Research·T MatsuhisaY Mori
Oct 1, 1990·Journal of the Neurological Sciences·T J BensteadP J Dyck
Jul 1, 1987·Surgical Neurology·Y ShimizuY Iwasaki
Jun 1, 1978·Prostaglandins·K PomerantzP Ramwell
Oct 1, 1978·Prostaglandins·C H Ts'aoC M Holly

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