Synthesis of ribosomal proteins during growth of Streptomyces coelicolor

Molecular Microbiology
G BlancoJ A Salas

Abstract

Changes in expression of ribosomal protein genes during growth and stationary phase of Streptomyces coelicolor A3(2) in liquid medium were studied. Proteins being synthesized were pulse-labelled with [35S]-methionine, separated by two-dimensional polyacrylamide gel electrophoresis, and quantified using the BioImage computer software. Most of the ribosomal proteins were synthesized throughout the life cycle. Exceptions were two proteins whose synthesis drastically decreased at the approach of stationary phase. These two proteins were identified in purified ribosomes as homologues of Escherichia coli ribosomal proteins L10 and L7/L12, using antibodies raised against fusion proteins between these ribosomal proteins and Escherichia coli beta-galactosidase. The genes (rplJ and rplL) encoding the L10 and L7/L12 proteins were contained in a 1.2 kb BamHI fragment that was cloned and sequenced. The linkage and order of the genes coincide with other L10-L7/L12 operons. However, L11 and L1 genes were not present immediately upstream of the L10 gene, as is the case for E. coli and other bacteria. Instead, two open reading frames of unknown function were found immediately upstream of the L10 gene, in an adjacent 1.9 kb BamHI fragment.

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Jan 7, 1977·Molecular & General Genetics : MGG·N E MurrayK Murray
Apr 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·L E PostP P Dennis
Nov 24, 1979·Nucleic Acids Research·H C Birnboim, J Doly
Jan 1, 1978·Journal of Bacteriology·M B Manzanal, C Hardisson
Sep 1, 1976·Journal of Bacteriology·C Hardisson, M B Manzanal
Dec 1, 1992·Canadian Journal of Microbiology·L M QuirósJ A Salas
Aug 1, 1992·Canadian Journal of Microbiology·I S NovellaJ Sánchez
Apr 1, 1992·Gene·F Wright, M J Bibb
Feb 1, 1991·Molecular Microbiology·E StrauchM J Bibb
Mar 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·B K LeskiwK F Chater
Jul 1, 1991·Journal of General Microbiology·V BascaránA F Braña
Dec 1, 1990·Electrophoresis·R A VanBogelenF C Neidhardt
Nov 1, 1990·Trends in Biochemical Sciences·M SarasteA Wittinghofer
Oct 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J A JaegerM Zuker
Feb 1, 1988·Molecular & General Genetics : MGG·H A Baylis, M J Bibb
Jan 1, 1970·Journal of General Microbiology·H Wildermuth, D A Hopwood
Jul 1, 1983·Journal of Bacteriology·K E Kendrick, J C Ensign
Nov 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·J GerminoD Bastia
Nov 1, 1984·Archives of Microbiology·R Koepsel, J C Ensign
Jan 11, 1984·Nucleic Acids Research·J DevereuxO Smithies

❮ Previous
Next ❯

Citations

Sep 2, 2003·Journal of Biotechnology·Juan F MartínMónica Barriuso
Dec 1, 1996·The Journal of Steroid Biochemistry and Molecular Biology·M E Baker
Apr 29, 2008·Applied and Environmental Microbiology·Angel MantecaJesus Sanchez
Jul 13, 2006·Annual Review of Biomedical Engineering·Kenneth R Diller

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Respiration

This feed focuses on cellular respiration in bacteria, known as bacterial respiration. Discover the latest research here.