PMID: 5771965Apr 1, 1969

Synthesis of Saint Louis encephalitis virus ribonucleic acid in BHK-21-13 cells

Journal of Virology
D W TrentA A Qureshi

Abstract

Infection of baby hamster kidney cells (BHK-21/13) with Saint Louis encephalitis (SLE) virus depressed the rate of protein and ribonucleic acid (RNA) synthesis until viral RNA synthesis began 6 hr postinfection (PI). Virus-directed RNA synthesis was subsequently inhibited until 12 hr PI when virion maturation began. The rate of protein synthesis reached a peak 6 hr PI and was subsequently depressed until just before the onset of virion maturation. Density gradient analysis of phenol-extracted RNA from actinomycin-treated infected cells indicated that, at 6 to 8 hr and again at 12 to 20 hr PI, three species of viral-specific RNA were synthesized. The most rapid sedimenting form (43S) was ribonuclease-sensitive and had a base composition similar to the RNA isolated from mature virions. The 20S RNA species was ribonuclease-resistant and had a sedimentation coefficient and base composition similar to the replicative form associated with other arbovirus infections. The 26S RNA was ribonuclease-resistant (0.2 mug/ml, 0.1 m NaCl, 25 C, 30 min) and had a nucleotide base composition closer to the 20S form than to the values for 43S RNA. Five-minute pulse labeling of infected cultures during the period viral RNA synthesis was maximal res...Continue Reading

Citations

Jan 1, 1972·Archiv für die gesamte Virusforschung·M Takehara
May 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Mohamed M Emara, Margo A Brinton
Jan 1, 1993·Archives of Virology·A I Bartholomeusz, P J Wright
Jan 1, 1977·Archives of Virology·T A BrawnerD W Trent
Jan 1, 1973·Archiv für die gesamte Virusforschung·R D CardiffP K Russell
Jul 1, 1971·Japanese Journal of Microbiology·C Nishimura, H Tsukeda

Related Concepts

Centrifugation, Density Gradient
Ac-De
DNA, Double-Stranded
Encephalitis Viruses
St. Louis Meningoencephalitis
Cricetus
Kidney
Leucine
Nucleotides
Phosphorus Isotopes

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