PMID: 7008029Dec 11, 1980Paper

Synthesis of the human insulin gene. Part III. Chemical synthesis of 5'-phosphomonoester group containing deoxyribooligonucleotides by the modified phosphotriester method. Its application in the synthesis of seventeen fragments constituting human insulin C-chain DNA

Nucleic Acids Research
H M HsiungS A Narang

Abstract

A method for phosphorylating a protected deoxyribooligonucleotide containing phosphotriester linkages is described. The modified phosphotriester method of chemical synthesis is further refined in terms of (i) better final deblocking conditions and (ii) new chromatography solvent systems containing acetone-water-ethyl acetate to yield pure oligomers. The effectiveness of these improvements has been demonstrated in the rapid and efficient synthesis of seventeen fragments constituting the sequence of human insulin C-chain DNA.

References

Dec 10, 1975·Journal of the American Chemical Society·N KatagiriS A Narang
Feb 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·A M Maxam, W Gilbert
Dec 10, 1975·Journal of the American Chemical Society·K ItakuraS A Narang
Jan 26, 1973·The Journal of Organic Chemistry·J C Catlin, F Cramer
Jul 1, 1965·Proceedings of the National Academy of Sciences of the United States of America·C C Richardson

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Citations

Jan 1, 1984·Pharmacology & Therapeutics·S A NarangF Georges
Oct 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·M IkeharaK Fujiyama
Nov 11, 1982·Nucleic Acids Research·E OhtsukaD Söll
Nov 11, 1982·Nucleic Acids Research·V A EfimovO G Chakhmakhcheva
Mar 11, 1988·Nucleic Acids Research·S R Presnell, S A Benner
Dec 11, 2007·Biotechnology Advances·Ai-Sheng XiongQuan-Hong Yao
Apr 1, 1986·Medicinal Research Reviews·S P Adams, G R Galluppi

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