Synthesis of the Na-K-ATPase gamma-subunit is regulated at both the transcriptional and translational levels in IMCD3 cells

American Journal of Physiology. Renal Physiology
J M CapassoT Berl

Abstract

We previously reported that hypertonicity-mediated upregulation of the gamma-subunit of Na-K-ATPase is dependent on both the JNK and the PI3 kinase pathways. The present experiments were undertaken to explore the mechanisms whereby these pathways regulate the expression of the gamma-subunit in inner medullary collecting duct cells (IMCD3). Inhibition of JNK with SP-600125 (20 muM), a concentration that causes an approximately 95% inhibition of hypertonicity-stimulated JNK activation, markedly decreased the amount of the gamma-subunit in response to 550 mosmol/kgH(2)O for 48 h. This was accompanied by a parallel decrease in the gamma-subunit mRNA. The rate at which the gamma-subunit mRNA decreased was unaffected by actinomycin D. In contrast, inhibition of PI3 kinase with LY-294002 results in a marked decrease in the amount of gamma-subunit protein but without alteration in gamma-subunit message. The rate at which the gamma-subunit protein decreased was unaffected by cyclohexamide. Transfection of IMCD3 cells with a gamma-subunit construct results in the expression of both gamma-subunit message and protein. However, in cortical collecting duct cells (M1 cells) such transfection resulted in expression of only the message and not ...Continue Reading

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Citations

Feb 8, 2006·Annual Review of Physiology·Haim Garty, Steven J D Karlish
Nov 5, 2008·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·F Y MaD J Nikolic-Paterson
Jan 13, 2006·American Journal of Physiology. Renal Physiology·Käthi Geering
Oct 20, 2006·American Journal of Physiology. Cell Physiology·Elena ArystarkhovaKathleen J Sweadner
Feb 8, 2008·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Christian Kølbaek Tipsmark
Aug 13, 2010·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Christian K TipsmarkSteffen S Madsen

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