PMID: 7372715May 1, 1980Paper

Synthesis of thylakoid membrane proteins by chloroplasts isolated from spinach. Cytochrome b559 and P700-chlorophyll a-protein

The Journal of Cell Biology
R E Zielinski, C A Price

Abstract

Intact chloroplasts, purified from spinach leaves by sedimentation in density gradients of colloidal silica, incorporate labeled amino acids into at least 16 different polypeptides of the thylakoid membranes, using light as the only source of energy. The thylakoid products of chloroplast translation were visualized by subjecting membranes purified from chloroplasts labeled with [35S]methionine to electrophoresis in high-resolution, SDS-containing acrylamide gradient slab gels and autoradiography. The apparent mol wt of the labeled products ranged from less than 10,000 to greater than 70,000. One of the labeled products is the apoprotein of the P700-chlorophyll a-protein (CPI). The CPI apoprotein is assembled into a pigment-protein complex which is electrophoretically indistinguishable from the native CPI complex. Isolated spinach chloroplasts also incorporate [3H]leucine and [35S]methionine into cytochrome b559. The radioactive label remains with the cytochrome through all stages of purification: extraction of the thylakoid membranes with Triton X-100 and urea, adsorption of impurities on DEAE cellulose, two cycles of electrophoresis in Triton-containing polyacrylamide gels and electrophoresis in SDS-containing gradient gels. C...Continue Reading

References

May 1, 1975·Archives of Biochemistry and Biophysics·J J MorgenthalerC A Price
Jul 1, 1979·European Journal of Biochemistry·A Doherty, J C Gray
Sep 1, 1978·The Journal of Cell Biology·A E GrebanierL Bogorad
Feb 1, 1976·FEBS Letters·L R Mendiola-MorgenthalerC A Price
Jan 1, 1976·Archives of Biochemistry and Biophysics·J J Morgenthaler, L Mendiola-Morgenthaler
Jul 28, 1967·Journal of Molecular Biology·S H Howell, E N Moudrianakis
Jan 3, 1974·European Journal of Biochemistry·H Weiss, B Ziganke
Sep 1, 1974·Archives of Biochemistry and Biophysics·W BottomleyP R Whitfeld
Dec 15, 1974·FEBS Letters·L R Mendiola-Morgenthaler, J J Morgenthaler
Mar 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·J Takemoto, J Lascelles
Jul 1, 1968·Archives of Biochemistry and Biophysics·J L Hedrick, A J Smith
Aug 1, 1959·Canadian Journal of Biochemistry and Physiology·E G BLIGH, W J DYER

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Citations

Jan 1, 1988·Photosynthesis Research·A GnanamR M Mannan
Mar 1, 1985·Plant Molecular Biology·P WesthoffR G Herrmann
Oct 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·A Watanabe, C A Price
Aug 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·D Bhaya, P A Castelfranco
Dec 1, 1983·The Journal of Cell Biology·E Vierling, R S Alberte
Oct 1, 1982·Plant Physiology·L E Fish, A T Jagendorf
Dec 1, 1984·Archives of Biochemistry and Biophysics·E Minami, A Watanabe
Feb 1, 1986·Archives of Biochemistry and Biophysics·E MinamiA Watanabe
Oct 15, 1983·Archives of Biochemistry and Biophysics·E M Reardon, C A Price

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