PMID: 6401638Jan 1, 1983Paper

Synthesis of thymosin alpha 1 precursor cDNA and purification of active mRNA by affinity chromatography

The International Journal of Biochemistry
J Rey-CamposM Freire

Abstract

1. Thymosin alpha 1 precursor [3H]cDNA-cellulose synthesis was carried out by reverse transcription with RNA-dependent DNA polymerase from avian myeloblastosis virus using oligo(dT)-bound to cellulose as a primer. 2. Unlabelled thymosin alpha 1 precursor cDNA-cellulose was synthetized in a preparative scale to be used in affinity chromatography. 3. Poly(A)-mRNA from calf thymus was subjected to cDNA-cellulose affinity chromatography and an active messenger obtained in a yield of 3% respect to the total thymus poly(A)-mRNA chromatographied. 3. The analysis of the translation products of the purified mRNA have shown it as the thymosin alpha 1 precursor messenger.

References

Feb 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·A L GoldsteinJ Meienhofer
Dec 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·M FreireB L Horecker
Oct 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·P Venetianer, P Leder
Jan 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·M FreireB L Horecker

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