PMID: 7518699Apr 1, 1994Paper

Synthesis of tissue factor pathway inhibitor in human synovial cells and chondrocytes makes joints the predilected site of bleeding in haemophiliacs

European Journal of Clinical Chemistry and Clinical Biochemistry : Journal of the Forum of European Clinical Chemistry Societies
T BrinkmannKnut Kleesiek

Abstract

The synthesis of tissue factor pathway inhibitor (TFPI) was investigated in cloned human synovial cells and human chondrocytes. TFPI-specific DNA transcription products of these cells were isolated, and a full-length cDNA of about 1000 base pairs was amplified by reverse transcription and polymerase chain reaction. The amplified DNA was cloned into the vector pUC 18. The TFPI coding sequence was confirmed by double stranded sequencing and was identical with that previously published for human TFPI coding nucleotide sequence from human placental cDNA (1). The inhibitory activity of TFPI in the cell medium of cultivated human chondrocytes and cloned human synovial cells was determined by a specific chromogenic substrate assay of factor Xa activity. The inhibitory activity of TFPI in the medium of human chondrocytes and cloned human synovial cells was 630-720 mU/10(8) cells and 1080-1665 mU/10(8) cells, respectively. In addition, TFPI activity in cell culture media of human chondrocytes and cloned human synovial cell was suppressed by a polyclonal goat anti-TFPI antibody directed against the inhibitory domain I and domain II. In the chromogenic substrate assay, the anti-TFPI antibody completely suppressed the inhibitory activity o...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Aug 1, 1992·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·A K LindahlU Abildgaard
Jan 1, 1988·CRC Critical Reviews in Biochemistry·R R Bach
Jan 1, 1985·Thrombosis Research·M C van Dam-MierasH C Hemker

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Citations

Jan 31, 2006·Molecular and Cellular Biochemistry·Elmar ThyzelChristian Götting
Mar 29, 2013·Haemophilia : the Official Journal of the World Federation of Hemophilia·S L SoodUNKNOWN HTCN Study Investigators
May 29, 2012·Journal of Thrombosis and Haemostasis : JTH·M E GorczycaA Srivastava
Apr 27, 2012·Haemophilia : the Official Journal of the World Federation of Hemophilia·Y DargaudC Négrier
May 31, 2012·Haemophilia : the Official Journal of the World Federation of Hemophilia·S OgawaK A Tanaka
Jan 24, 2006·Seminars in Hematology·W Keith Hoots
Jul 5, 2005·Clinica Chimica Acta; International Journal of Clinical Chemistry·Elmar ThyzelKnut Kleesiek
Apr 22, 2017·Haemophilia : the Official Journal of the World Federation of Hemophilia·L F D van VulpenR E G Schutgens

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