PMID: 36442May 1, 1979

Synthesis of two classes of antibody, gammaM and gammaG or gammaM and gammaA, by identical cells. Amplification of the antibody response to pneumococcal polysaccharide type III

The Journal of Experimental Medicine
V J PasanenP J Baker

Abstract

Class-specific plaque-forming cell (PFC) (gammaM, gamma1, gamma2, and gammaA) responses to type III pneumococcal polysaccharide (SSS-III) were studied in BALB/c x C57BL/6F1 (CBF1) mice with and without induction of an allogeneic effect. Gamma1, gamma2, and gammaA PFC were detected in two ways: (a) With the sequential development of the assay slides, first for direct (gammaM)PFC followed by incubation with class-specific antiimmunoglobulin and complement for the development of additional gamma1, gamma2, and gammaA PFC (gammaM-independent gamma1, gamma2, and gammaA PFC); and (b) by blocking gammaM PFC with goat anti-gammaM and simultaneously developing gamma1, gamma2, and gammaA PFC (total gamma1-, gamma2-, and gammaA-secreting PFC). The results showed that whereas gammaM PFC arose on the 3rd d after immunization, gamma1-, gamma2-, and gammaA-secreting PFC arose on the 4th to 5th d after immunization. They appeared in association with gammaM-secreting PFC because they were detected with the gammaM blocking method but not with the sequential method. By the 7th d most gamma1, gamma2, and gammaA PFC were detected by the sequential method as well, indicating that those antibodies were secreted independently of cells secreting gammaM....Continue Reading

References

Feb 28, 1975·Annals of the New York Academy of Sciences·E WeckerL Kühn
Jun 1, 1973·The Journal of Experimental Medicine·P J BakerB Prescott
Feb 1, 1972·The Journal of Experimental Medicine·A R LawtonM D Cooper
Jun 1, 1972·The Journal of Experimental Medicine·D D Manning, J W Jutila
Mar 1, 1964·The Journal of Experimental Medicine·G J NOSSALC M AUSTIN

Citations

Sep 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·P J GearhartJ J Cebra
Feb 1, 1983·Scandinavian Journal of Immunology·D A RivierM F Kagnoff
Feb 1, 1984·Scandinavian Journal of Immunology·V J Pasanen, O Mäkelä

Related Concepts

Antibodies, Bacterial
Antibody-Secreting Cells
Antigens, Bacterial
IgA2
Polyglobin
IgM2
Mice, Inbred Strains
Polysaccharides, Bacterial
Streptococcus pneumoniae

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