PMID: 6989604Mar 1, 1980Paper

Synthesis of types I, III and AB2 collagen by chick tendon fibroblasts in vitro

European Journal of Biochemistry
H HerrmannK von der Mark

Abstract

Tendons from 14--17-day-old chick embryos contain predominantly type I collagen and about 5% AB2 collagen; type III collagen is not detectable by biochemical methods, such as sodium dodecyl sulfate/polyacrylamide gel electrophoresis or cyanogen bromide pattern, but can be visualized by immunofluorescence staining with collagen-type-specific antibodies. Similarly, freshly dissociated tendon cells secrete only type I collagen into the culture medium but no significant amounts of type III collagen [Uitto, J., Lichtenstein, J. R., and Bauer, E. A. (1976) Biochemistry, 15, 4935--4942]. Transfer of tendon cells from chick embryos to monolayer conditions, however, initiated synthesis of type III collagen in about 10% of the cells within three days, as visualized by immunofluorescence staining. Secretion of type III collagen into the culture medium can also be detected by sodium dodecyl sulfate/polyacrylamide gel electrophoresis. With increasing number of passages the number of cells producing type III collagen reached levels of about 80% after the third passage, while 90% of all cells stained positively for type I collagen. This is reflected by an increase of production of type III collagen as determined by CM-cellulose chromatography...Continue Reading

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Citations

Jan 1, 1988·Archives of Dermatological Research·K KatsuokaT Krieg
May 1, 1985·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·A GaugerM A Riederer-Henderson
Jul 1, 1984·Developmental Biology·M WongR Stern
Aug 1, 1991·The Journal of Hand Surgery : Journal of the British Society for Surgery of the Hand·G A MurrellL Bromley
Apr 15, 1981·Developmental Biology·J SasseK von der Mark
Jul 1, 1982·Collagen and Related Research·H Birkedal-HansenR Gay
Oct 1, 1992·The Journal of Hand Surgery : Journal of the British Society for Surgery of the Hand·S O AbrahamssonL S Lohmander
Dec 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·S L FriedmanD M Bissell
Nov 1, 1987·The Journal of Cell Biology·D R KeeneR E Burgeson
Dec 1, 1993·Journal of Periodontology·L T Hou, J A Yaeger
Jun 1, 1981·Experimental Eye Research·G W ConradH F Edelhauser
May 1, 1983·Journal of Periodontal Research·H LimebackJ E Aubin
Jan 1, 1985·Annals of the New York Academy of Sciences·T KriegR Fleischmajer
Jan 1, 1986·Differentiation; Research in Biological Diversity·J Mollenhauer, K Bayreuther
Jun 24, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Anne E C NicholsAlayna E Loiselle
Aug 1, 1986·The Journal of Investigative Dermatology·L WeberT Krieg
Oct 1, 1985·The Journal of Investigative Dermatology·A AlbiniP Mueller
May 1, 1981·Experimental Cell Research·M WiestnerP K Müller
Mar 1, 1988·Journal of the American Academy of Dermatology·T Krieg, M Meurer

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