Synthesis, Structural and Micromechanical Properties of 3D Hyaluronic Acid-Based Cryogel Scaffolds

Biomacromolecules
Claude OelschlaegerNorbert Willenbacher

Abstract

In this study, macroporous, elastic, three-dimensional scaffolds formed of hyaluronic acid mixed with ethylene glycol diglycidyl ether as a chemical cross-linker have been prepared by cryogelation for application in tissue engineering. These cryogels are characterized by large interconnected pores of size ∼50-300 μm and pore wall thickness of ∼5-30 μm as determined from confocal microscopy images. Variation of pH, freezing temperature, and polymerization time allows for control of pore size and shape as well as matrix thickness. These structural properties then determine mechanical strength as well as swelling capacity. Furthermore, increasing hyaluronic acid concentration decreases cryogel pore size, reduces swelling properties, and reinforces mechanical properties. On the other hand, decreasing cross-linker concentration, at a constant hyaluronic acid concentration, increases pore size and swelling capacity but provides less rigidity. Additionally, for the first time, local elastic properties of the polymer matrix and viscous properties of the pores have been characterized using multiple particle tracking microrheology. Local matrix elasticity, relaxation time of hyaluronic acid chains, and the degree of heterogeneity are dis...Continue Reading

References

Oct 23, 1997·Journal of Oral and Maxillofacial Surgery : Official Journal of the American Association of Oral and Maxillofacial Surgeons·H NeoA N Goss
Jun 11, 1998·Trends in Biotechnology·B S Kim, D J Mooney
Nov 22, 2001·The Journal of Biological Chemistry·Eva A TurleyLilly Y W Bourguignon
Nov 22, 2001·The Journal of Biological Chemistry·Bryan P TooleMarkku I Tammi
Sep 27, 2002·Biomaterials·R BarbucciR Giardino
Sep 5, 2003·Postgraduate Medical Journal·I UthmanB Haraoui
Sep 27, 2003·Trends in Biotechnology·Vladimir I LozinskyBo Mattiasson
Apr 2, 2004·Journal of Biomedical Materials Research. Part a·D M Ebenstein, L A Pruitt
Jul 9, 2004·Biochimica Et Biophysica Acta·Vincent C HascallThomas N Wight
Nov 10, 2004·Biophysical Journal·Thierry Savin, Patrick S Doyle
Jan 11, 2005·Biomacromolecules·Iuliana GatejMarguerite Rinaudo
Nov 19, 2005·Science·Dennis E DischerYu-Li Wang
May 1, 1986·The Biochemical Journal·E A BalazsR W Jeanloz
Aug 23, 2006·Cell·Adam J EnglerDennis E Discher
Jul 4, 2008·Journal of Materials Science. Materials in Medicine·Era JainAshok Kumar
Aug 15, 2008·Biomacromolecules·Nadja NijenhuisJos A E Spaan
Oct 7, 2008·Trends in Microbiology·Fatima M PlievaBo Mattiasson
May 1, 2009·Acta Biomaterialia·Tzu-Wei Wang, Myron Spector
Jul 22, 2009·Journal of Biomaterials Science. Polymer Edition·Jing Di ChenXiaofeng Chen
Jun 16, 2010·Journal of Biomaterials Science. Polymer Edition·Young-Gwang KoGuoping Chen
Jul 10, 2010·International Journal of Biological Sciences·Deepti SinghAshok Kumar
Aug 10, 2010·Journal of Biomedical Materials Research. Part a·Nihal Engin VranaGarrett B McGuinness
Nov 29, 2011·Colloids and Surfaces. B, Biointerfaces·Hyung-Don HwangDae-Duk Kim
Dec 2, 2011·Journal of Colloid and Interface Science·Dmitriy BerilloHarald Kirsebom
Aug 14, 2012·Journal of Bioscience and Bioengineering·Sumrita Bhat, Ashok Kumar
Jan 25, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Shawn C OwenMolly S Shoichet
Aug 1, 2012·Materials Science & Engineering. C, Materials for Biological Applications·Marina Flaibani, Nicola Elvassore
Jan 15, 2014·Materials Science & Engineering. C, Materials for Biological Applications·Senta ReicheltMatthias Schnabelrauch
Jan 15, 2014·Tissue Engineering. Part a·Stephanie A FisherMolly S Shoichet
Apr 22, 2014·Interface Focus·Mark Ahearne
Dec 11, 2014·Materials Science & Engineering. C, Materials for Biological Applications·Archana SharmaAshok Kumar
Jun 7, 2013·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Timothy M A HendersonAndrea J O'Connor

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Citations

Oct 30, 2016·International Journal of Biological Macromolecules·S ThönesU Anderegg
Jan 16, 2020·Soft Matter·Federica BurlaGijsje H Koenderink
Aug 9, 2018·Materials·Mahboobeh RezaeeyazdiSidi A Bencherif
Nov 10, 2017·Der Ophthalmologe : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft·M ThallerF Geiger
Sep 16, 2017·Soft Matter·Li-Chiun ChengPatrick S Doyle
Jan 12, 2021·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Ugo MasulloMarta Madaghiele
Aug 3, 2021·Annals of Plastic Surgery·Ozgur HurkalCemile Merve Seymen

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