Synthesized basement membranes direct the differentiation of mouse embryonic stem cells into pancreatic lineages

Journal of Cell Science
Yuichiro HiguchiShoen Kume

Abstract

We previously reported that embryonic stem (ES) cells cultured on M15 cells, a mesoderm-derived supportive cell line, were efficiently differentiated towards an endodermal fate, finally adopting the specific lineages of various digestive organs such as the pancreas and liver. We show here that the endoderm-inducing activity of M15 cells is in part mediated through the extracellular matrices, and that laminin alpha5 is one of the crucial components. In an attempt to establish a feeder-free ES-cell procedure for pancreatic differentiation, we used a synthesized basement membrane (sBM) substratum using an HEK293 cell line stably expressing laminin-511. On the sBM, mouse ES or induced pluripotent stem (iPS) cells sequentially differentiated into the definitive endoderm, pancreatic progenitor cells, and then insulin-expressing pancreatic beta-cells in vitro. Knockdown of ES cells with integrin beta1 (Itgb1) reduces differentiation towards pancreatic cells. Heparan sulfate proteoglycan 2 (HSPG2) knockdown and heparitinase treatment synergistically decreased the number of Pdx1-expressing cells. These findings indicate that components of the basement membrane have an important role in the differentiation of definitive endoderm lineages...Continue Reading

References

Apr 1, 1990·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·P D Yurchenco, J C Schittny
Jan 1, 1997·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·J Taipale, J Keski-Oja
Jun 8, 2000·Developmental Dynamics : an Official Publication of the American Association of Anatomists·H Colognato, P D Yurchenco
Oct 22, 2002·American Journal of Physiology. Endocrinology and Metabolism·Manami HaraGraeme I Bell
Jun 5, 2003·Developmental Biology·Konstantin I IzvolskyWellington V Cardoso
Oct 28, 2003·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Christiana Ruhrberg
Oct 29, 2003·Current Opinion in Biotechnology·Hynda K KleinmanMatthew P Hoffman
Nov 7, 2003·The Journal of Biological Chemistry·Zhengliang L WuRobert D Rosenberg
Mar 3, 2005·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Takumi TerataniTakahiro Ochiya
Nov 29, 2005·Nature Biotechnology·Masahiro YasunagaShin-Ichi Nishikawa
Jun 8, 2007·Nature·Keisuke OkitaShinya Yamanaka
Dec 7, 2007·Nature Biotechnology·Masato NakagawaShinya Yamanaka
Dec 15, 2007·Nature Protocols·Kazutoshi TakahashiShinya Yamanaka
Jun 3, 2008·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Nobuaki ShirakiShoen Kume
Jul 17, 2008·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Kathryn D RodgersOlena Jacenko
Mar 3, 2009·Biochemical and Biophysical Research Communications·Nobuaki ShirakiShoen Kume
Apr 2, 2009·Biochemical and Biophysical Research Communications·Iwao TakahashiAkira Sugawara

❮ Previous
Next ❯

Citations

Jul 4, 2012·Current Diabetes Reports·Insa S Schroeder
Mar 23, 2011·Cold Spring Harbor Perspectives in Biology·Peter D Yurchenco
Dec 1, 2012·Stem Cells Translational Medicine·Philippe A LysySusan Bonner-Weir
Dec 10, 2013·Nature Chemical Biology·Daisuke SakanoShoen Kume
Jun 8, 2010·Cell Structure and Function·Nobuaki ShirakiShoen Kume
Feb 18, 2016·Acta Biomaterialia·D F Braga MaltaG H Underhill
Nov 10, 2010·Expert Opinion on Biological Therapy·Takashi HoshibaGuoping Chen
Oct 31, 2015·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Ryutaro NakashimaShoen Kume
Jan 24, 2012·Biotechnology Advances·Kirthanashri Srinivasan VasanthanSwaminathan Sethuraman
Jun 30, 2012·Current Opinion in Genetics & Development·Marine R C Kraus, Anne Grapin-Botton
Oct 19, 2012·Cell Adhesion & Migration·Caroline SpenléJeffrey H Miner
Jun 2, 2016·BMC Developmental Biology·Soichiro OgakiShoen Kume
Oct 16, 2015·Biochemistry Insights·Han WangJake Leighton
Jul 11, 2014·Nihon yakurigaku zasshi. Folia pharmacologica Japonica·Shoen Kume
Mar 17, 2017·American Journal of Physiology. Cell Physiology·Taku KaitsukaKazuhito Tomizawa
Nov 30, 2014·Biomaterials Science·Binata JoddarYoshihiro Ito
Jun 21, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·T Hoshiba
Dec 22, 2020·Frontiers in Bioengineering and Biotechnology·Marisa AssunçãoAnna Blocki
Apr 25, 2015·Journal of Cell Science·Hermes Taylor-WeinerAdam J Engler

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.