Synthesized peptide 710-725 from HCV subtype 1a E2 glycoprotein blocks HCV infection through competitive binding of CD81

International Journal of Molecular Medicine
Xiaojing LiuMin Liu

Abstract

Hepatitis C virus (HCV) infection is a significant public health problem worldwide. However, there is still a lack of effective therapeutic drugs which could be used for the interruption of the disease. In the present study, for the first time, we reported that a synthesized peptide, which was synthesized by solid phase peptide synthesis and derived from the amino acids 710 to 725 of the HCV E2, functioned as an inhibitor of HCV infection. Using an MTT assay, we found that the E2 (710-725) peptide exerted no specific cytotoxicity on Huh7.5 cells and primary human hepatocytes (pHH). Interestingly, E2 (710-725) peptide blocked the entry of cell culture‑derived HCV (HCVcc) into hepatocytes. Moreover, it suppressed HCV RNA replication and HCV-specific protein NS3 and NS5B expression, as shown by western blot analysis. Moreover, E2 (710-725) markedly attenuated the inhibitory effect of HCVcc on hepatocyte viability. Additionally, a co-immunino-precipitation assay demonstrated that E2 (710-725) abrogated the interaction between CD81 and HCV E2 envelope protein through competitive binding of CD81. Overall, our results revealed that the synthesized peptide E2 (710-725) blocked CD81‑mediated HCV entry and possessed the potential to trea...Continue Reading

References

Oct 30, 1998·Science·P PileriS Abrignani
Jul 15, 2000·Seminars in Liver Disease·A Wasley, M J Alter
Mar 5, 2003·The Journal of Experimental Medicine·Birke BartoschFrançois-Loïc Cosset
Jan 15, 2004·Journal of Virology·Jie ZhangJane A McKeating
Jun 9, 2005·Proceedings of the National Academy of Sciences of the United States of America·Jin ZhongFrancis V Chisari
Jun 11, 2005·Science·Brett D LindenbachCharles M Rice
Oct 18, 2005·Journal of Virology·Zhen-Yong KeckSteven K H Foung
Oct 19, 2007·Journal of Virology·Sonia MolinaChantal Fournier-Wirth
Nov 26, 2008·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Philip MeulemanGeert Leroux-Roels
Jan 14, 2010·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Sophie E KriegerThomas F Baumert
Jan 22, 2010·Journal of Virology·Yong-Yuan ZhangT Jake Liang
Dec 15, 2010·Journal of Virology·Anna AlbeckaJean Dubuisson
Jun 10, 2011·Nature·Marcus DornerAlexander Ploss
Feb 16, 2012·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Shufeng LiuTianyi Wang
Mar 7, 2012·Reviews in Medical Virology·Luke W MeredithJane A McKeating
Mar 30, 2012·The Journal of Infectious Diseases·Marcel KrepstakiesUlrike Protzer
Apr 24, 2013·The New England Journal of Medicine·Ira M JacobsonUNKNOWN FUSION Study
May 6, 2014·The New England Journal of Medicine·Stefan ZeuzemUNKNOWN VALENCE Investigators
Sep 15, 2014·The Journal of Infection·Radwa Yehia MekkyAhmed Ihab Abdelaziz
Nov 8, 2014·International Journal of Peptide Research and Therapeutics·Terence N BukongGyongyi Szabo
Nov 25, 2014·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Changhua JiKlaus Klumpp
Mar 21, 2015·Current Opinion in Virology·Abdul Ghafoor KhanJoseph Marcotrigiano

❮ Previous
Next ❯

Citations

Dec 24, 2018·Retrovirology·Benjamin J Patters, Santosh Kumar

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.