Synthetic biodegradable microparticles for articular cartilage tissue engineering

Journal of Biomedical Materials Research. Part a
H ThissenJ A Werkmeister

Abstract

Articular cartilage tissue engineering procedures require the transplantation of chondrocytes that have been expanded in vitro. The expansion is carried out for a considerable time and can lead to a modulation of cell phenotype. However, microcarrier cultures have been shown to allow cell expansion while maintaining the phenotype. Here, we have used the biodegradable polyester poly(lactide-co-glycolide) (PLGA) in the form of microspheres and irregular shaped microparticles with a diameter between 47 and 210 microm. Surface modification of particles was carried out by ammonia plasma treatment and subsequent adsorption of collagen. Alternatively, particles were modified by partial hydrolysis and subsequent immobilization of an amine-terminated dendrimer. Each surface modification step was characterized by X-ray photoelectron spectroscopy. The effectiveness of the surface modification procedures was demonstrated by in vitro cell culture experiments using sheep articular cartilage chondrocytes. A significant influence of both the particle shape and the surface chemistry on the proliferation rate was observed while the phenotype was maintained independent of the surface chemistry or particle shape. Chondrocytes cultured on PLGA micr...Continue Reading

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Citations

Nov 13, 2008·Cytotechnology·Tracy A TebbJerome A Werkmeister
Jun 10, 2010·Chemical Communications : Chem Comm·Rodney T ChenFrank Caruso
Apr 23, 2008·Pediatric Research·Daniel S Kohane, Robert Langer
May 8, 2007·Advanced Drug Delivery Reviews·Hyun Jung Chung, Tae Gwan Park
May 16, 2009·Journal of Tissue Engineering and Regenerative Medicine·Sofia PetterssonGunnar Kratz
Aug 31, 2010·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Emily AnglinNicolas H Voelcker
Feb 23, 2017·ACS Applied Materials & Interfaces·Fanyi LiJohn S Forsythe
Oct 5, 2010·Cell Transplantation·Li-Yi SunTzyy-Wen Chiou
Sep 26, 2009·Biomedical Materials·A Champa Jayasuriya, Archana Bhat
Jun 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Charlotte A HenshawPhilip M Williams
Dec 22, 2011·The Journal of Physical Chemistry. B·Dewi P GoAndrea J O'Connor

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