Sep 9, 2008

Synthetic biology with RNA motifs

The International Journal of Biochemistry & Cell Biology
Hirohide Saito, Tan Inoue

Abstract

Structural motifs in naturally occurring RNAs and RNPs can be employed as new molecular parts for synthetic biology to facilitate the development of novel devices and systems that modulate cellular functions. In this review, we focus on the following: (i) experimental evolution techniques of RNA molecules in vitro and (ii) their applications for regulating gene expression systems in vivo. For experimental evolution, new artificial RNA aptamers and RNA enzymes (ribozymes) have been selected in vitro. These functional RNA molecules are likely to be applicable in the reprogramming of existing gene regulatory systems. Furthermore, they may be used for designing hypothetical RNA-based living systems in the so-called RNA world. For the regulation of gene expressions in living cells, the development of new riboswitches allows us to modulate the target gene expression in a tailor-made manner. Moreover, recently RNA-based synthetic genetic circuits have been reported by employing functional RNA molecules, expanding the repertory of synthetic biology with RNA motifs.

  • References68
  • Citations26
  • References68
  • Citations26

Citations

Mentioned in this Paper

Tetracycline Antibiotics
RNA Aptamers
Tetracyclines
Biochemical Pathway
Conserved Sequence
RNA Conformation
Transcription Repressor/Corepressor
RNA Ligase (ATP)
RNA, Untranslated
Enzymes, antithrombotic

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