Synthetic cannabinoid BB-22 (QUCHIC): Human hepatocytes metabolism with liquid chromatography-high resolution mass spectrometry detection

Journal of Pharmaceutical and Biomedical Analysis
Jeremy CarlierMarilyn A Huestis

Abstract

Clandestine laboratories continue producing new synthetic cannabinoids that mimic and magnify natural cannabinoids effects to circumvent drug scheduling legislation. New synthetic cannabinoids are highly potent and responsible for many acute intoxications and deaths. Characterization of metabolic pathways is critical to identify metabolite markers whose detection can prove intake. BB-22 is a new potent synthetic cannabinoid whose toxicological and metabolic properties are currently unavailable. Analytical methods require constant updating and are challenging due to extensive synthetic cannabinoid metabolism and low marker concentrations. A single non-specific BB-22 metabolite was previously identified in incubations with human liver microsomes (BB-22 3-carboxyindole). Clear characterization of BB-22's metabolism is required to help toxicologists document BB-22 consumption in clinical and forensic cases. We incubated 10 μmol/L BB-22 with cryopreserved human hepatocytes for 3 h. Samples were analyzed by liquid chromatography on a biphenyl column and high resolution mass spectrometry. Results were processed with data mining software, identifying ten metabolites. Loss of the quinolinyl side-chain via ester hydrolysis was the main b...Continue Reading

Citations

Dec 16, 2018·The Journal of Pharmacology and Experimental Therapeutics·Thomas F GamageBrian F Thomas
Apr 2, 2020·Journal of Analytical Toxicology·Kayoko MinakataKanako Watanabe
Jul 12, 2018·Drug Testing and Analysis·Per Ole M GundersenMartin Josefsson
Mar 20, 2019·Frontiers in Chemistry·Xingxing Diao, Marilyn A Huestis
Jan 1, 2021·International Journal of Molecular Sciences·Jeremy CarlierMarilyn A Huestis
Jan 2, 2021·Forensic Science International : Synergy·Wing-Sum ChanBobbie Kwok-Keung Cheung

❮ Previous
Next ❯

Related Concepts

Related Feeds

Absence Epilepsy

Absence epilepsy is a common seizure disorder in children which can produce chronic psychosocial sequelae. Discover the latest research on absence epilepsies here.

Addiction

This feed focuses mechanisms underlying addiction and addictive behaviour including heroin and opium dependence, alcohol intoxication, gambling, and tobacco addiction.