Synthetic, Context-Dependent Microbial Consortium of Predator and Prey

ACS Synthetic Biology
Feng LiuQiang Hua

Abstract

Synthetic microbial consortia are a rapidly growing area of synthetic biology. So far, most consortia are designed without considering their environments; however, in nature, microbial interactions are constantly modulated by cellular contexts, which, in principle, can dramatically alter community behaviors. Here we present the construction, validation, and characterization of an engineered bacterial predator-prey consortium that involves a chloramphenicol (CM)-mediated, context-dependent cellular interaction. We show that varying the CM level in the environment can induce success in the ecosystem with distinct patterns from predator dominance to prey-predator crossover to ecosystem collapse. A mathematical model successfully captures the essential dynamics of the experimentally observed patterns. We also illustrate that such a dependence enriches community dynamics under different initial conditions and further test the resistance of the consortium to invasion with engineered bacterial strains. This work exemplifies the role of the context dependence of microbial interactions in modulating ecosystem dynamics, underscoring the importance of including contexts into the design of engineered ecosystems for synthetic biology applic...Continue Reading

References

Nov 10, 1995·The Journal of Biological Chemistry·O P KuipersW M de Vos
Jun 12, 2004·The Journal of Dairy Research·Antonio FernándezJuan M Rodríguez
Dec 25, 2004·Science·Phillip S CoburnMichael S Gilmore
Dec 19, 2006·Gene·Michael J CallananTodd R Klaenhammer
Feb 3, 2007·Proceedings of the National Academy of Sciences of the United States of America·Wenying ShouJose M G Vilar
Jun 7, 2007·Proceedings of the National Academy of Sciences of the United States of America·Wilfried WeberMartin Fussenegger
Apr 17, 2008·Molecular Systems Biology·Frederick K BalagaddéLingchong You
Aug 5, 2008·Trends in Biotechnology·Katie BrennerFrances H Arnold
Jan 19, 2010·Nature Chemical Biology·Adam C PalmerRoy Kishony
Sep 9, 2010·Molecular Systems Biology·Edwin H Wintermute, Pamela A Silver
Dec 3, 2010·PLoS Computational Biology·Niels Klitgord, Daniel Segrè
Dec 15, 2010·The ISME Journal·Herwig BachmannJohan E T van Hylckama Vlieg
Jul 17, 2012·Nature Reviews. Microbiology·Karoline Faust, Jeroen Raes
Mar 19, 2014·Current Opinion in Microbiology·Tobias Grosskopf, Orkun S Soyer
Apr 30, 2014·Proceedings of the National Academy of Sciences of the United States of America·Michael T MeeHarris H Wang
May 23, 2014·ACS Synthetic Biology·Wentao Kong, Ting Lu
Apr 29, 2015·Journal of Bacteriology·Angela T NguyenAmanda G Oglesby-Sherrouse
Sep 1, 2015·Current Opinion in Biotechnology·Stephanie G HaysPamela A Silver
Sep 6, 2015·Applied and Environmental Microbiology·Tobias SpießKarl-Dieter Entian
Oct 28, 2015·Nucleic Acids Research·Wentao KongTing Lu
Feb 20, 2016·The ISME Journal·Damian W RivettThomas Bell
Feb 21, 2016·Applied and Environmental Microbiology·Nemanja MirkovicMilan Kojic
Mar 12, 2016·The ISME Journal·Stephen R LindemannAlexander S Beliaev
Apr 17, 2016·Current Opinion in Microbiology·Nathan I JohnsHarris H Wang
May 20, 2016·Proceedings of the National Academy of Sciences of the United States of America·Eugene Anatoly YurtsevJeff Gore
May 28, 2016·Current Opinion in Microbiology·Otto X Cordero, Manoshi S Datta
Nov 30, 2016·The ISME Journal·Breah LaSarreJames B McKinlay
Nov 14, 2017·Nature Chemical Biology·Fernando VillarrealCheemeng Tan
Mar 15, 2018·PLoS Biology·Christoph Ratzke, Jeff Gore
Mar 27, 2018·Current Opinion in Microbiology·Philip BittihnJeff Hasty
Jun 27, 2018·Nature Chemical Biology·Wentao KongTing Lu
Nov 27, 2018·Science Advances·Venhar Celik OzgenTing Lu

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Citations

Aug 15, 2020·Frontiers in Bioengineering and Biotechnology·Behzad D KarkariaAlex J H Fedorec
Jan 18, 2020·Nature Communications·Feng LiuTing Lu
Jan 30, 2021·Nature Communications·Behzad D KarkariaChris P Barnes
Nov 18, 2020·Current Opinion in Biotechnology·Kent M RappMichael J Betenbaugh

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