Synthetic copolymer 1 and myelin basic protein do not require processing prior to binding to class II major histocompatibility complex molecules on living antigen-presenting cells

Cellular Immunology
M Fridkis-HareliM Sela

Abstract

In the present study we attempted to examine whether copolymer 1 (Cop 1), a synthetic basic random copolymer of amino acids (a candidate drug for multiple sclerosis (MS)), and myelin basic protein (MBP) undergo processing prior to their binding to MHC class II molecules on antigen-presenting cells (APC). The direct binding of biotinylated Cop 1 and MBP to living APC was monitored by flow cytometry using phycoerythrin (PE)-streptavidin. The time course for either Cop 1 or MBP binding was similar at 37 degrees C and on ice. Both Cop 1 and MBP bound to glutaraldehyde-fixed APC. Furthermore, these biotinylated antigens bound also in the presence of protease inhibitors and lysosomotropic agents, suggesting that proteolysis is not required prior to their interaction with the MHC determinants. Finally, short fragments of Cop 1 molecule did not bind to most of the APC, suggesting that the polymeric nature of Cop 1 is important for its efficient and promiscuous binding.

Citations

Apr 13, 2006·Rejuvenation Research·Michael Sela
Dec 11, 2013·Clinical & Developmental Immunology·Masha Fridkis-Hareli
Oct 15, 1998·Proceedings of the National Academy of Sciences of the United States of America·M Fridkis-HareliJ L Strominger
Aug 17, 2004·Proceedings of the National Academy of Sciences of the United States of America·Michael Sela, Edna Mozes
Dec 30, 1999·International Reviews of Immunology·M Sela
Apr 25, 2013·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Yu YouRuxiang Xu
Jan 21, 2004·Journal of Molecular Recognition : JMR·Ruth Arnon, Michael Sela
Jul 31, 2007·European Journal of Neurology : the Official Journal of the European Federation of Neurological Societies·R EngelsteinR Gabizon
Oct 5, 2001·Expert Opinion on Pharmacotherapy·M Sela, D Teitelbaum
May 18, 1999·International Immunology·M Fridkis-HareliJ L Strominger
Mar 4, 2000·The Journal of Biological Chemistry·A O TzianabosD L Kasper
Jan 7, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·W M Kalka-MollD L Kasper
Aug 21, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kazuyuki KawamuraThomas G Forsthuber
Oct 14, 2018·CNS Drugs·Stefanie KuertenTimothy L Vollmer

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