Synthetic gene recruitment reveals adaptive reprogramming of gene regulation in yeast

Genetics
Elad StolovickiErez Braun

Abstract

The recruitment of a gene to a foreign regulatory system is a major evolutionary event that can lead to novel phenotypes. However, the evolvability potential of cells depends on their ability to cope with challenges presented by gene recruitment. To study this ability, we combined synthetic gene recruitment with continuous culture and online measurements of the metabolic and regulatory dynamics over long timescales. The gene HIS3 from the histidine synthesis pathway was recruited to the GAL system, responsible for galactose utilization in the yeast S. cerevisiae. Following a switch from galactose to glucose--from induced to repressed conditions of the GAL system--in histidine-lacking chemostats (where the recruited HIS3 is essential), the regulatory system reprogrammed to adaptively tune HIS3 expression, allowing the cells to grow competitively in pure glucose. The adapted state was maintained for hundreds of generations in various environments. The timescales involved and the reproducibility of separate experiments render spontaneous mutations an unlikely underlying mechanism. Essentially all cells could adapt, excluding selection over a genetically variable population. The results reveal heritable adaptation induced by the ex...Continue Reading

References

Nov 5, 1981·Journal of Molecular Biology·K Struhl, R W Davis
Jun 1, 1995·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·D LohrJ Zlatanova
Mar 1, 1996·Microbiological Reviews·P AlifanoC B Bruni
Oct 7, 1996·Journal of Theoretical Biology·H Kacser, J R Small
Jul 22, 1998·Proceedings of the National Academy of Sciences of the United States of America·M Kirschner, J Gerhart
Sep 22, 1998·Origins of Life and Evolution of the Biosphere : the Journal of the International Society for the Study of the Origin of Life·R FaniA Lazcano
Mar 18, 1999·Current Genetics·A MariniG Morpurgo
May 14, 1999·Current Opinion in Microbiology·M Carlson
Sep 10, 1999·Journal of Theoretical Biology·C Pál, I Miklós
Oct 6, 2000·Methods : a Companion to Methods in Enzymology·Y ItoY Imanishi
Oct 19, 2000·Methods in Enzymology·J Horecka, G F Sprague
Dec 5, 2000·Molecular Biology of the Cell·A P GaschP O Brown
Feb 17, 2001·Molecular Biology of the Cell·H C CaustonR A Young
Jun 19, 2001·The EMBO Journal·S R Biggar, G R Crabtree
Mar 22, 2002·Nature·Lars M SteinmetzRonald W Davis
Jul 27, 2002·Annual Review of Cell and Developmental Biology·John R True, Sean B Carroll
Jan 2, 2003·Nature Genetics·Suzanne L Rutherford, Steven Henikoff
Dec 1, 1950·Proceedings of the National Academy of Sciences of the United States of America·A NOVICK, L SZILARD
Jul 9, 2004·Molecular Biology of the Cell·Alok J SaldanhaDavid Botstein
Feb 22, 2005·Nature Genetics·Ran KafriYitzhak Pilpel
May 13, 2005·Nature·Murat AcarAlexander van Oudenaarden
Jul 8, 2005·PLoS Biology·Sean B Carroll

❮ Previous
Next ❯

Citations

Aug 26, 2010·Molecular Biotechnology·Chen-Hsiang Yeang
Apr 17, 2013·Molecular Biology and Evolution·Lior DavidErez Braun
Jan 16, 2008·PLoS Computational Biology·Chikara Furusawa, Kunihiko Kaneko
Jun 24, 2011·PloS One·Elad Stolovicki, Erez Braun
May 15, 2013·PloS One·Chikara Furusawa, Kunihiko Kaneko
Dec 7, 2013·PloS One·Lindsay S MooreErez Braun
Jul 21, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Lamont S TangEve Marder
Jul 2, 2008·Nature Reviews. Genetics·Luis López-MauryJürg Bähler
Jun 3, 2014·The Journal of Physiology·Ehud Lamm
Jun 26, 2009·Chemistry & Biodiversity·Piet Herdewijn, Philippe Marlière
Apr 25, 2007·Molecular Systems Biology·Shay SternErez Braun
May 27, 2011·Molecular Systems Biology·Saburo TsuruTetsuya Yomo
Aug 18, 2010·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Adam S Wilkins
Apr 23, 2015·Studies in History and Philosophy of Biological and Biomedical Sciences·Ulrich Krohs
Apr 24, 2015·Studies in History and Philosophy of Biological and Biomedical Sciences·Sara Green
Apr 24, 2015·Studies in History and Philosophy of Biological and Biomedical Sciences·Erez Braun, Shimon Marom
Aug 20, 2014·Biochimica Et Biophysica Acta·M ElgartY Soen
Dec 23, 2011·Journal of Biomedicine & Biotechnology·Yuki MatsumotoTetsuya Yomo
Jul 4, 2012·Theoretical Population Biology·E FilibaN Brenner
Sep 15, 2012·Progress in Biophysics and Molecular Biology·Eva Jablonka
Oct 2, 2012·Progress in Biophysics and Molecular Biology·Keith Baverstock
Jan 8, 2015·Nucleic Acids Research·Yoshie MurakamiTetsuya Yomo
Apr 22, 2017·Nature Communications·Hallel I SchreierNaama Brenner
Apr 2, 2017·Biochemical and Biophysical Research Communications·Bei-Wen YingTetsuya Yomo
Oct 6, 2006·Physical Biology·Naama BrennerErez Braun
Feb 1, 2017·The EMBO Journal·Aneta Koseska, Philippe Ih Bastiaens
Dec 3, 2008·IET Systems Biology·K Kaneko, C Furusawa
Oct 29, 2013·Physical Biology·Yair KatzirErez Braun
Feb 27, 2015·Reports on Progress in Physics·Erez Braun
Jan 18, 2020·Scientific Reports·Yusuke Himeoka, Kunihiko Kaneko
May 7, 2020·Proceedings of the National Academy of Sciences of the United States of America·Gabrielle WoronoffJérôme Bibette

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.