Synthetic glycopeptides as a designated standard in focused glycoproteomics to discover serum cancer biomarkers

MedChemComm
K V YogeshShin-Ichiro Nishimura

Abstract

Previous studies on the large-scale glycomics of more than 3500 human serum samples revealed that the serum glycoproteins of cancer patients often have more dominant and specific glycoforms, namely, branched tri- and tetra-antennary N-glycans, most cancer patient groups than normal control groups. We herein established an efficient synthetic protocol of glycopeptides having highly complicated N-glycan structures that may be generated by direct tryptic digestion of serum glycoproteins. A preliminary selected reaction monitoring (SRM) assay using the synthetic model glycopeptide 1, 40Ser-Val-Gln-Glu-Ile-Gln-Ala-Thr-Phe-Phe-Tyr-Phe-Thr-Pro-Asn-Lys-Thr-Glu-Asp-Thr-Ile-Phe-Leu-Arg63 having an asialo tri-antennary N-glycan at the Asn54 residue as a designated calibration standard allowed for the rapid and absolute quantitation of the tryptic fragment derived from the serum α1-acid glycoprotein carrying a focused N-glycoform of cancer patients and healthy controls in a range between 200 and 1600 fmole μL-1 without any enrichment process for the target glycoprotein.

References

Jul 20, 1973·Science·C B Anfinsen
Dec 19, 2002·Molecular & Cellular Proteomics : MCP·N Leigh Anderson, Norman G Anderson
Aug 15, 2003·Molecular & Cellular Proteomics : MCP·Radhakrishna S TirumalaiTimothy D Veenstra
Oct 13, 2006·Annual Review of Immunology·James N ArnoldRaymond A Dwek
Sep 14, 2007·Annual Review of Physiology·Christine L Hattrup, Sandra J Gendler
Jan 8, 2008·Cell Metabolism·Ralph J DeBerardinisCraig B Thompson
Oct 20, 2009·Nature Methods·Jonas NilssonGöran Larson
Nov 6, 2009·Journal of the American Chemical Society·Ping WangSamuel J Danishefsky
Nov 26, 2009·Nature Reviews. Cancer·Donald W Kufe
Jan 13, 2010·Cell·James W DennisMichael Demetriou
Jun 24, 2010·Molecular & Cellular Proteomics : MCP·Masaki KurogochiShin-Ichiro Nishimura
Jul 19, 2011·Advances in Carbohydrate Chemistry and Biochemistry·Shin-Ichiro Nishimura
Feb 7, 2012·Journal of Proteome Research·William R AlleyMilos V Novotny
May 4, 2012·Journal of the American Chemical Society·Jonathan J GoodfellowBenjamin G Davis
Jul 4, 2012·Journal of the American Chemical Society·Wei HuangLai-Xi Wang
Jan 12, 2013·Journal of Gastroenterology·Kazuhiro NousoKazuhide Yamamoto
Jan 17, 2013·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Toshiya KamiyamaSatoru Todo
Jun 5, 2013·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Koji MiyaharaKazuhide Yamamoto
Nov 23, 2013·Organic Letters·H V Ravi KumarShin-Ichiro Nishimura
Oct 29, 2014·Expert Review of Proteomics·Robert Harlan, Hui Zhang
Feb 26, 2015·Journal of Physiology and Biochemistry·Zhumin LuoDing-Feng Su
Feb 3, 2016·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Frano VučkovićGordan Lauc
Mar 10, 2016·Biochimica Et Biophysica Acta·Juan Etxebarria, Niels-Christian Reichardt
May 3, 2016·Cell Systems·Philipp E GeyerMatthias Mann
Jun 30, 2016·Journal of Proteome Research·Kazuhiro TanabeSadayo Iijima
Jul 23, 2016·Angewandte Chemie·Manuel MönnichCarlo Unverzagt

❮ Previous
Next ❯

Citations

Dec 13, 2018·Journal of Agricultural and Food Chemistry·Jurgen T SanesShin-Ichiro Nishimura

❮ Previous
Next ❯

Methods Mentioned

BETA
glycosylation

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.