Synthetic high-density lipoprotein nanoconjugate targets neuroblastoma stem cells, blocking migration and self-renewal

Surgery
Chitra SubramanianMark S Cohen

Abstract

Pathways critical for neuroblastoma cancer stem cell function are targeted by 4,19,27-triacetyl withalongolide A (WGA-TA). Because neuroblastoma cells and their cancer stem cells highly overexpress the scavenger receptor class B type 1 receptor that binds to synthetic high-density lipoprotein, we hypothesized that a novel mimetic synthetic high-density lipoprotein nanoparticle would be an ideal carrier for the delivery of 4,19,27-triacetyl withalongolide to neuroblastoma and neuroblastoma cancer stem cells. Expression of scavenger receptor class B type 1 in validated human neuroblastoma cells was evaluated by quantitative polymerase chain reaction (qPCR) and Western blot. In vitro cellular uptake of synthetic high-density lipoprotein nanoparticles was observed with a fluorescence microscope. In vivo biodistribution of synthetic high-density lipoprotein nanoparticles was investigated with IVIS imaging. Self-renewal and migration/invasion were assessed by sphere formation and Boyden chamber assays, respectively. Viability was analyzed by CellTiter-Glo assay. Cancer stem cell markers were evaluated by flow cytometry. qPCR and Western blot analysis revealed a higher level of scavenger receptor class B type 1 expression and drug upt...Continue Reading

References

Apr 17, 2004·Journal of Pediatric Hematology/oncology·Loretta LauDavid Malkin
Apr 23, 2008·Cancer·Victor M SantanaCatherine A Billups
Aug 8, 2009·Cancer Treatment Reviews·Fazlul H Sarkar, Yiwei Li
Jan 8, 2010·Molecular Cancer Therapeutics·Srinivas KoduruChendil Damodaran
Nov 22, 2011·Journal of Natural Products·Huaping ZhangBarbara N Timmermann
Aug 28, 2012·Advanced Drug Delivery Reviews·Marina G DamianoC Shad Thaxton
Sep 1, 2012·Anti-cancer Agents in Medicinal Chemistry·Michael L MegisonElizabeth A Beierle
Oct 6, 2012·Chemical & Pharmaceutical Bulletin·Huaping ZhangBarbara Timmermann
Dec 21, 2012·Journal of Natural Products·Huaping ZhangBarbara N Timmermann
Jan 25, 2013·Proceedings of the National Academy of Sciences of the United States of America·Shuo YangC Shad Thaxton
May 23, 2013·Expert Opinion on Emerging Drugs·Victoria CastelPablo Berlanga
Jan 25, 2014·Frontiers in Pediatrics·Nirupama SabnisAndras G Lacko
Apr 26, 2014·World Journal of Surgery·Chitra SubramanianMark Cohen
Dec 2, 2014·Pediatric Clinics of North America·Meredith S Irwin, Julie R Park
Dec 10, 2014·Expert Review of Anticancer Therapy·Linda FoitColby Shad Thaxton
Feb 19, 2016·ACS Nano·Rui KuaiAnna Schwendeman
Sep 28, 2016·Advances in Experimental Medicine and Biology·Peter T WhiteMark S Cohen
Dec 7, 2016·International Journal of Nanomedicine·Yue YuanAnna Schwendeman
Apr 25, 2017·Nanomedicine : Nanotechnology, Biology, and Medicine·Jie TangAnna Schwendeman
Sep 19, 2017·International Journal of Nanomedicine·Rui KuaiAnna Schwendeman

❮ Previous
Next ❯

Citations

Jan 13, 2019·The Journal of Pharmacology and Experimental Therapeutics·Carlos Rodríguez-NogalesMaría J Blanco-Prieto
Sep 25, 2020·Journal of Controlled Release : Official Journal of the Controlled Release Society·Dennis Pedersbæk, Jens B Simonsen

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

ApoE, Lipids & Cholesterol

Serum cholesterol, triglycerides, apolipoprotein B (APOB)-containing lipoproteins (very low-density lipoprotein (VLDL), immediate-density lipoprotein (IDL), and low-density lipoprotein (LDL), lipoprotein A (LPA)) and the total cholesterol/high-density lipoprotein (HDL) cholesterol ratio are all connected in diseases. Here is the latest research.

Related Papers

Journal of B.U.ON. : Official Journal of the Balkan Union of Oncology
Han-Liang JiangWei-Dong Han
Hepatology : Official Journal of the American Association for the Study of Liver Diseases
M A Arrese, J M Crawford
Biochemical and Biophysical Research Communications
Marlyn Panchoo, Andras G Lacko
© 2022 Meta ULC. All rights reserved