PMID: 1242563Nov 1, 1975Paper

Synthetic human calcitonin: analysis of antibodies obtained from various animal species and determination of immunoreactive hormone in human sera

Acta Endocrinologica
F M DietrichJ A Fischer

Abstract

Antibodies to synthetic human calcitonin (hCT) were developed in rabbits, goats and mice. The free peptide (32 amino-acid residues, Mwt. 3418) was administered together with adjuvant, and the effect of various immunization procedures, as well as of different dose-levels, was evaluated comparatively. Synthetic hCT was found to be a good immunogen for the three animal species examined. The relative importance of various structural parts of the hCT molecule with regard to immunological specificity was determined by reference to the inhibition of the specific binding of 125I-hCT to antibodies by peptide fragments of hCT. All the antisera studied were directed to structural and/or conformational properties of the 11-28 or 11-32 amino acid sequence of hCT. Six different antisera from rabbits and goats were selected for radioimmunological assay of hCT on the basis of their inhibitory dose50-values and immunological specificity. To improve the sensitivity of the radioimmunoassay (RIA), we studied the preparation of radioiodinated hCT and assessed various parameters determining the sensitivity of the assay. Despite all the efforts, CT in human plasma from healthy subjects could not be determined with certainty. The difficulties encounte...Continue Reading

Citations

Jan 1, 1988·Annales d'endocrinologie·P Sai, J F Chatal
Feb 1, 1991·Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·R MuffJ A Fischer
Jan 1, 1992·European Journal of Clinical Pharmacology·C BeglingerJ A Fischer
Aug 1, 1977·Clinica Chimica Acta; International Journal of Clinical Chemistry·S Rojanasathit, J G Haddad
Aug 1, 1985·Regulatory Peptides·G RümenapfP O Schwille
Aug 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·J MoranJ A Fischer
Dec 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·J A FischerJ B Martin
Jul 15, 1978·Klinische Wochenschrift·F RaueR Ziegler
Mar 1, 1984·Clinical Endocrinology·P H ToblerJ A Fischer
Apr 1, 1982·Clinical Endocrinology·V Lo CascioL A Scuro
Mar 1, 1994·Scandinavian Journal of Gastroenterology·G RümenapfD Galewski
Jan 1, 1978·Acta Radiologica: Oncology, Radiation, Physics, Biology·K J OlsenA Johannsen
Jan 1, 1979·Acta Radiologica: Oncology, Radiation, Physics, Biology·P ThommesenP Funch-Jensen
Jan 1, 1980·Acta Radiologica. Oncology·L Wählby, G Westman
Dec 16, 1977·Mycopathologia·G C Llewellyn, C E O'Rear
Jan 1, 1979·Acta Medica Scandinavica·H E NielsenK J Olsen
Jan 1, 1980·Acta Medica Scandinavica·K EmmertsenH H Hansen
Dec 1, 1979·Acta Orthopaedica Scandinavica·H E NielsenO Elbrønd
Dec 1, 1989·The American Journal of Physiology·M P GnaedingerJ A Fischer
Jan 1, 1979·The American Journal of Physiology·R HuwylerJ A Fischer
Nov 1, 1980·The American Journal of Physiology·P WielandA Huch
Mar 30, 1988·Biochemical and Biophysical Research Communications·S Dotti-SigristJ A Fischer

❮ Previous
Next ❯

Related Concepts

Related Feeds

Avian Influenza: Innate Immune Adjuvant

Adjuvants systems that are added to vaccines against avian influenza have be explored to enhance the innate immune system response against the virus. Here is the latest research on avian influenza and the innate immune adjuvant.

Avian Influenza: Innate Immune Adjuvant (ASM)

Adjuvants systems that are added to vaccines against avian influenza have be explored to enhance the innate immune system response against the virus. Here is the latest research on avian influenza and the innate immune adjuvant.

Antibody Specificity

Antibodies produced by B cells are highly specific for antigen as a result of random gene recombination and somatic hypermutation and affinity maturation. As the main effector of the humoral immune system, antibodies can neutralize foreign cells. Find the latest research on antibody specificity here.