Synthetic low-calcaemic vitamin D(3) analogues inhibit secretion of insulin-like growth factor II and stimulate production of insulin-like growth factor-binding protein-6 in conjunction with growth suppression of HT-29 colon cancer cells

Molecular and Cellular Endocrinology
Y S OhJ H Park

Abstract

The aims of the present study were to compare the ability of various synthetic analogues of 1 alpha,25-dihydroxyvitamin D(3) [1 alpha,25-(OH)(2)D(3)] to inhibit proliferation of HT-29 cells, a human colon adenocarcinoma cell line. HT-29 cells were incubated for 144 h with various concentrations (0-100 nM) of 1 alpha,25-(OH)(2)D(3), or the analogues EB1089, CB1093 or 1 beta,25-(OH)(2)D(3). All these analogues except 1 beta,25-(OH)(2)D(3) inhibited cell proliferation, but relative potencies and efficacies of EB1089 and CB1093 were much greater than that of the native vitamin. Cells grew in serum-free medium, reaching a plateau density at day 10 of culture, and addition of 10 nM 1 alpha,25-(OH)(2)D(3) or 1 beta,25-(OH)(2)D(3) did not alter the long-term growth characteristics of HT-29 cells. However, cells treated with 10 nM EB1089 or CB1093 grew at a rate slower than control and reached final densities that were 53+/-1 and 36+/-2% lower than control, respectively. Immunoblot analysis of serum-free conditioned medium using a monoclonal anti-insulin-like growth factor-(IGF)-II antibody showed that both 10 nM EB1089 and CB1093 markedly inhibited secretion of both mature 7500 M(r) and higher M(r) forms of IGF-II. Ligand blot and immu...Continue Reading

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Citations

Jan 15, 2005·International Journal of Colorectal Disease·Rajaraman DuraiMarc C Winslet
May 26, 2005·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·Leon A Bach
Mar 3, 2005·The Journal of Nutritional Biochemistry·Woo K KimJung H Y Park
Mar 3, 2012·Endocrine-related Cancer·Fábio PereiraAlberto Muñoz
Oct 2, 2013·Endocrine-related Cancer·Callum Livingstone
Aug 15, 2013·Critical Reviews in Oncology/hematology·Michelino Di RosaLucia Malaguarnera
Jun 2, 2016·The British Journal of Nutrition·Ruoxu DouShuji Ogino
Nov 7, 2012·Clinical Science·Leon A BachZhiyong Yang
Apr 10, 2015·Pharmaceutical Biology·Marco GiammancoGaetano Leto
Apr 5, 2015·Digestive Diseases and Sciences·Myong Ki BaegSang-Woo Kim
Apr 30, 2014·Frontiers in Endocrinology·Andreas HoeflichJulia Brenmoehl
Sep 14, 2016·Oncotarget·Qiuyan ChenYi Lu
May 8, 2020·European Journal of Clinical Investigation·George CiuleiLucia Maria Procopciuc
Jul 8, 2018·The Journal of Steroid Biochemistry and Molecular Biology·Gemma Ferrer-MayorgaAlberto Muñoz

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