Synthetic miR-16-5p does not act as a reverse transcriptase co-factor to enhance detection of small RNA

BioRxiv : the Preprint Server for Biology
Melissa A McAlexander, Kenneth W Witwer

Abstract

Failure to detect low-abundance microRNAs (miRNAs), for example, in circulating plasma, may occur for a variety of reasons, including presence of enzyme inhibitors. Recently, we received the unusual but intriguing suggestion that miR-16-5p acts as a co-factor of reverse transcriptases, facilitating more efficient reverse transcription of miRNAs and thus enhanced detection of low-abundance miRNAs. We tested this hypothesis by incubating reverse transcriptase with several concentrations of synthetic miR-16-5p and then performing stem-loop RT-qPCR with serial dilutions of miRNA osa-MIR168a. Our results do not support a role for miR-16 as a co-factor of reverse transcriptase.

Related Concepts

Real-Time Polymerase Chain Reaction
Enzyme Inhibitor Drugs
Small Nuclear RNA
MicroRNA Gene
DNA-Directed RNA Polymerase
MIRN16 microRNA, human
Transcriptase
Reverse Transcriptase Polymerase Chain Reaction
Quantitative Reverse Transcriptase PCR
Plasma

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