Synthetic miRNA sponges driven by mutant hTERT promoter selectively inhibit the progression of bladder cancer

Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine
Chengle ZhuangZhiming Cai

Abstract

The mutant promoter of human telomerase reverse transcriptase (hTERT) shows high transcriptional activity in bladder cancer cells. Some up-regulated microRNAs (miRNAs) are reported as oncogenic factors in bladder cancer. Previous studies report that miRNAs can be inhibited by base-pairing interactions. The purpose of this study is to construct a synthetic device driven by mutant hTERT promoter to suppress four up-regulated miRNAs and to verify its effects on phenotypes of bladder cancer cells and human normal cells. Tandem bulged miRNA binding sites targeting oncogenic miRNAs were inserted into the 3' untranslated region (3' UTR) of mutant hTERT promoter-driven Renilla luciferase gene to construct a synthetic tumor-specific device, miRNA sponges. A negative control was generated by using tandem repeated sequences without targeting any known miRNA. Bladder cancer cells (T24, 5637, UM-UC-3) and human fiber cells (HFC) were transfected with devices. Various functional assays were used to detect the effects of this device. The activity of the mutant hTERT promoter detected by luciferase assay was about three times as large as the wild-type hTERT promoter in bladder cancer cells, while it could not be measured in HFC. Other assays i...Continue Reading

References

Mar 21, 2001·Human Molecular Genetics·J W ShayW E Wright
Jun 10, 2005·Nature·Lin HeScott M Hammond
Jun 22, 2007·Nature·Ramiro E Verdun, Jan Karlseder
Aug 19, 2007·Nature Methods·Margaret S EbertPhillip A Sharp
Dec 20, 2007·Molecular Systems Biology·Luis Serrano
Feb 8, 2008·Nature Reviews. Cancer·Calvin B Harley
Apr 2, 2008·Cancer Science·Wun-Jae Kim, Suk-Chul Bae
Nov 19, 2010·Molecular Biology Reports·Aneta WojtylaBlazej Rubis
Jan 14, 2011·Nature Reviews. Urology·Johannes Falke, J Alfred Witjes
Jul 28, 2011·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Marta GladychBlazej Rubis
Nov 26, 2011·Expert Review of Anticancer Therapy·Amy Q LeiChong-xian Pan
Feb 23, 2012·Urologia Internationalis·M RacioppiP F Bassi
May 5, 2012·International Braz J Urol : Official Journal of the Brazilian Society of Urology·Gustavo Nader MartaHeloisa de Andrade Carvalho
Aug 11, 2012·European Urology·Maximilian BurgerYair Lotan
Jan 26, 2013·Science·Susanne HornRajiv Kumar
Jan 26, 2013·Science·Franklin W HuangLevi A Garraway
May 29, 2013·Nature Reviews. Urology·Hirofumi YoshinoHideki Enokida
Jul 4, 2013·Genetic Testing and Molecular Biomarkers·Shan ZhaoNan Ding
Aug 13, 2013·Trends in Biochemical Sciences·Kee Chung Low, Vinay Tergaonkar
Oct 9, 2013·Proceedings of the National Academy of Sciences of the United States of America·P Sivaramakrishna RachakondaRajiv Kumar
Jun 17, 2014·FEBS Letters·Zhiyong YanShusheng Che
Jul 13, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Tiantian WangYaoting Gui

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Citations

May 22, 2016·Journal of Experimental & Clinical Cancer Research : CR·Yonghao ZhanWeiren Huang
Nov 1, 2017·Journal of Experimental & Clinical Cancer Research : CR·Chengle ZhuangYaoting Gui
Jan 10, 2020·Current Topics in Medicinal Chemistry·Joseph BereiNeelu Puri
Sep 8, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Zachary SchrankNeelu Puri

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