Synthetic mRNA capping.

Beilstein Journal of Organic Chemistry
Fabian MuttachAndrea Rentmeister

Abstract

Eukaryotic mRNA with its 5'-cap is of central importance for the cell. Many studies involving mRNA require reliable preparation and modification of 5'-capped RNAs. Depending on the length of the desired capped RNA, chemical or enzymatic preparation - or a combination of both - can be advantageous. We review state-of-the art methods and give directions for choosing the appropriate approach. We also discuss the preparation and properties of mRNAs with non-natural caps providing novel features such as improved stability or enhanced translational efficiency.

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Citations

Feb 16, 2019·Chembiochem : a European Journal of Chemical Biology·Nils MuthmannAndrea Rentmeister
Sep 25, 2019·Vaccines·Leroy VersteegJeroen Pollet
Oct 19, 2019·Chembiochem : a European Journal of Chemical Biology·Josef LeiterRonald Micura
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Methods Mentioned

BETA
in vitro transcription
using in vitro
surface plasmon resonance
FRET
affinity purification

Software Mentioned

mScript

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