Synthetic OCP heterodimers are photoactive and recapitulate the fusion of two primitive carotenoproteins in the evolution of cyanobacterial photoprotection

The Plant Journal : for Cell and Molecular Biology
Sigal Lechno-YossefCheryl A Kerfeld

Abstract

The orange carotenoid protein (OCP) governs photoprotection in the majority of cyanobacteria. It is structurally and functionally modular, comprised of a C-terminal regulatory domain (CTD), an N-terminal effector domain (NTD) and a ketocarotenoid; the chromophore spans the two domains in the ground state and translocates fully into the NTD upon illumination. Using both the canonical OCP1 from Fremyella diplosiphon and the presumably more primitive OCP2 paralog from the same organism, we show that an NTD-CTD heterodimer forms when the domains are expressed as separate polypeptides. The carotenoid is required for the heterodimeric association, assembling an orange complex which is stable in the dark. Both OCP1 and OCP2 heterodimers are photoactive, undergoing light-driven heterodimer dissociation, but differ in their ability to reassociate in darkness, setting the stage for bioengineering photoprotection in cyanobacteria as well as for developing new photoswitches for biotechnology. Additionally, we reveal that homodimeric CTD can bind carotenoid in the absence of NTD, and name this truncated variant the C-terminal domain-like carotenoid protein (CCP). This finding supports the hypothesis that the OCP evolved from an ancient fusi...Continue Reading

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Citations

Jul 5, 2017·The New Phytologist·Cheryl A KerfeldMaria Agustina Dominguez-Martin
Sep 19, 2019·Nature Plants·Fernando MuzzopappaDiana Kirilovsky
Sep 25, 2020·Scientific Reports·Maria Agustina Dominguez-MartinCheryl A Kerfeld
Sep 18, 2020·Biomolecular NMR Assignments·Eugene G MaksimovVladimir I Polshakov
Mar 11, 2018·Biochemistry. Biokhimii︠a︡·N N SluchankoE G Maksimov
Nov 18, 2019·Biochimica Et Biophysica Acta. Bioenergetics·Valentyna KuznetsovaTomáš Polívka
Mar 19, 2019·Current Opinion in Structural Biology·Pimkhuan Hannanta-AnanBrian Y Chow
Mar 23, 2019·Current Opinion in Structural Biology·Maria Agustina Dominguez-Martin, Cheryl A Kerfeld
Jun 23, 2019·Biochimica Et Biophysica Acta. Bioenergetics·Chakib DjediatCécile Bernard
May 22, 2020·The Journal of Physical Chemistry. B·Tuhin KhanTomáš Polívka

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