Abstract
A 15-mer synthetic peptide, designated P1, was derived from the bone morphogenetic protein (BMP) receptor I and BMP receptor II binding domains of BMP-2 for the purpose of enhancing bone regeneration capacity of inorganic bovine bone mineral. A second peptide, denoted P2, was designed by adding seven glutamic acid residues to the N-terminal of P1 to increase the surface coating efficiency onto bone mineral. The coating efficiency of P1 increased with the concentration of peptide. P2 peptide, in contrast, had a higher coating efficiency at lower peptide concentrations. The peptides properly transduced intracellular signals properly via the Smad and ERK pathways, thereby increasing mineralization in vitro, implying that the peptides alone can induce osteoblastic differentiation. Adhesion of cells to bone mineral was greater when peptides were present than in bone mineral alone. P1- and P2-coated bone mineral increased osteoblastic differentiation, as demonstrated by ALPase activity. P1-coated bone mineral stimulated more new bone regeneration in bone defect sites after 2 weeks than the peptide-free control. These peptides, in combination with bone grafts or implants, have the potential to enhance osteoblastic differentiation and ...Continue Reading
References
Oct 15, 1990·Biochemical and Biophysical Research Communications·T KatagiriT Suda
Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·A J CelesteJ M Wozney
Dec 1, 1994·The Journal of Cell Biology·T KatagiriT Suda
Nov 1, 1993·Annals of Plastic Surgery·S R ThallerH Tesluk
Jan 4, 1996·Biochimica Et Biophysica Acta·R FujisawaY Kuboki
Aug 1, 1996·Journal of Biomedical Materials Research·J J Qian, R S Bhatnagar
Sep 23, 1997·Biomaterials·M E Nimni
Apr 7, 1998·Nature Biotechnology·A H Reddi
Jul 11, 1998·Journal of Biomedical Materials Research·J A KoempelD M Toriumi
Aug 1, 1998·Journal of Controlled Release : Official Journal of the Controlled Release Society·Y J ParkS J Lee
Feb 20, 1999·Journal of Cellular Biochemistry·E HayP J Marie
Apr 30, 1999·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·J M SchmittJ O Hollinger
Feb 19, 2000·Biochemical and Biophysical Research Communications·J LouP R Manske
May 10, 2000·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·S KasugaiK Ohya
Jul 6, 2000·Nature Structural Biology·T KirschM K Dreyer
Nov 14, 2000·Molecular and Cellular Biology·K S LeeS C Bae
Feb 24, 2001·Journal of Biomedical Materials Research·S ItohK Shinomiya
May 10, 2001·Bone·S GalleaS Roman-Roman
Jan 5, 2002·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Guozhi XiaoRenny T Franceschi
Jan 5, 2002·Journal of Controlled Release : Official Journal of the Controlled Release Society·Jue-Yeon LeeSeung-Jin Lee
Aug 2, 2002·Bone·R NishimuraT Yoneda
Nov 7, 2002·International Journal of Oral and Maxillofacial Surgery·H Schilephake
Dec 21, 2002·The Journal of Biological Chemistry·Coralee E TyeHarvey A Goldberg
Sep 23, 2003·Biochimica Et Biophysica Acta·Atsuhiro SaitoMasao Tanihara
Oct 19, 2004·Journal of Bone and Mineral Metabolism·Kousuke IbaToshihiko Yamashita
Mar 1, 2006·Journal of Biomedical Materials Research. Part a·Yang-Jo SeolChong-Pyoung Chung
Citations
Dec 23, 2011·International Journal of Endocrinology·Fabiana Viegas RaimundoTania Weber Furlanetto
Feb 1, 2013·Journal of Tissue Engineering and Regenerative Medicine·Xue ZhangFu-Zhai Cui
Feb 9, 2012·Connective Tissue Research·Jeong Joon YooHee Joong Kim
Sep 11, 2012·Journal of Biomedical Materials Research. Part a·Young Suk ChoiYoon Jeong Park
Jun 15, 2012·Journal of Biomedical Materials Research. Part a·Po-Chun ChangChi-Hwa Wang
Aug 29, 2012·Journal of Biomedical Materials Research. Part a·Yoon Jung ChoiYoon Jeong Park
Jul 20, 2016·Stem Cells International·Jonathan W LoweryVicki Rosen
Feb 26, 2016·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Wei CuiXiaodong Guo
Jan 1, 2011·Journal of Functional Biomaterials·Juliana T Y LeeWai-Hung Tsang
May 6, 2011·Journal of Periodontology·Chang-Kyun LeeChong-Pyoung Chung