Synthetic peptides as structural maquettes of Angiotensin-I converting enzyme catalytic sites.

Bioinorganic Chemistry and Applications
Zinovia SpyrantiPaul Cordopatis

Abstract

The rational design of synthetic peptides is proposed as an efficient strategy for the structural investigation of crucial protein domains difficult to be produced. Only after half a century since the function of ACE was first reported, was its crystal structure solved. The main obstacle to be overcome for the determination of the high resolution structure was the crystallization of the highly hydrophobic transmembrane domain. Following our previous work, synthetic peptides and Zinc(II) metal ions are used to build structural maquettes of the two Zn-catalytic active sites of the ACE somatic isoform. Structural investigations of the synthetic peptides, representing the two different somatic isoform active sites, through circular dichroism and NMR experiments are reported.

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Methods Mentioned

BETA
circular
NMR
Circular Dichroism
X-ray

Software Mentioned

Bruker
XWIN
NMR
DYANA
CALIBA
Linux
XEASY
CDNN CD Spectra Deconvolution

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