Synthetic peptides corresponding to various hydrophilic regions of the large subunit of cytochrome b558 inhibit superoxide generation in a cell-free system from neutrophils

Biochemical and Biophysical Research Communications
M Y ParkS Kanegasaki

Abstract

Cytochrome b558 is a component of the superoxide-generating system in neutrophils and plays key roles in both the assembly of a functional complex with cytosolic proteins and shuttling an electron from NADPH to molecular oxygen. To determine the role of predicted hydrophilic domains of gp91-phox, a glycosylated subunit of the cytochrome, we synthesized peptides corresponding to the regions and tested whether they affected superoxide generation in the cell-free system obtained from human neutrophils. Among twelve peptides tested, six peptides, four of which correspond to previously unreported regions, inhibited superoxide generation in the cell-free system. All of the active peptides were effective when added to the system before activation with sodium dodecyl sulfate. Four peptides, including two peptides corresponding to two newly identified regions, inhibited the translocation of the cytosolic components, p47-phox and p67-phox. The extent of inhibition on translocation of these components varied depending on the peptide used.

References

Sep 15, 1977·Journal of Molecular Biology·P Y Chou, G D Fasman
Aug 14, 1992·Biochemical and Biophysical Research Communications·H SumimotoS Minakami
Sep 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·B D VolppR A Clark
May 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·C A ParkosS H Orkin
May 5, 1982·Journal of Molecular Biology·J Kyte, R F Doolittle
Jul 18, 1995·Proceedings of the National Academy of Sciences of the United States of America·F R DeLeoM T Quinn
Oct 25, 1994·Proceedings of the National Academy of Sciences of the United States of America·T L LetoI de Mendez
Jun 7, 1994·Proceedings of the National Academy of Sciences of the United States of America·H SumimotoK Takeshige

❮ Previous
Next ❯

Citations

Jun 2, 2011·Nature Reviews. Drug Discovery·Grant R DrummondChristopher G Sobey
Feb 9, 2010·The Journal of Biological Chemistry·Heather M JacksonJ David Lambeth
Aug 21, 2010·The Journal of Biological Chemistry·Franck DebeurmeMarie-José Stasia
Jan 27, 2012·The Journal of Biological Chemistry·Katharina von LöhneysenUlla G Knaus
Dec 9, 2008·Antioxidants & Redox Signaling·Ina HelmckeRalf P Brandes
Mar 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·P M DangB M Babior
May 9, 2012·Cellular and Molecular Life Sciences : CMLS·Jamel El-BennaAxel Périanin
May 16, 2012·Cellular and Molecular Life Sciences : CMLS·Eugenia Cifuentes-PaganoPatrick J Pagano
Aug 5, 2004·Histochemistry and Cell Biology·William M Nauseef
Oct 4, 2006·The Journal of Biological Chemistry·Ross M TaylorAlgirdas J Jesaitis
Jun 10, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·James B BurrittAlgirdas J Jesaitis
Jun 1, 2010·Biochemical Pharmacology·Jamel El-BennaAxel Périanin

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.