Synthetic peptides representing sequences within gp41 of HIV as immunogens for murine T- and B-cell responses

Archives of Virology
L E BrownD C Jackson

Abstract

Within the gp41 glycoprotein of the human immunodeficiency virus type 1 (HIV-1) there is a relatively conserved region which appears accessible to the immune system during the course of HIV infection and is recognised by antibody from virtually all patients with AIDS. This region has also been shown to function as a target for human T cells. We have examined synthetic peptides spanning this sequence, between residues 572 and 604, with a view to evaluating their potential as immunogens. Peptides 572GIKQLQARILAVERYLKDQQ591 and 579RILAVERYLKDQQLLGGIWGCSGK601 were good immunogens in two different strains of mice while peptide 576LQARILAVERYLKDQQ591 was an inferior immunogen, and peptide 593LGIWGCSGKLIC604 was non-immunogenic unless coupled to a carrier protein. For both antibody and T cell responses it was apparent that sequences that could function as determinants within one peptide could not do so in the context of a different peptide immunogen. It follows that by judicious choice of immunogen sequence it may be possible to direct the immune response towards a desired fine specificity. Unwanted responses by CD4+ T cells isolated from certain peptide-primed animals were also observed. These T cells showed an unusual reactivity in ...Continue Reading

References

Jan 1, 1992·Clinical and Experimental Immunology·S J BellR Penny
Nov 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·C WildT Matthews
Jun 1, 1990·Vaccine·G Goodman-SnitkoffR J Mannino
Nov 6, 1990·Journal of Immunological Methods·N J MaejiH M Geysen
Aug 1, 1991·The Journal of Experimental Medicine·Q J Sattentau, J P Moore
Jan 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·M GirardE Muchmore
Apr 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·M K GornyS Zolla-Pazner
Mar 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·D R MilichA Moriarty
Mar 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·T J PalkerB F Haynes
May 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J R RuscheP J Fischinger
Jun 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J GoudsmitD C Gajdusek
Dec 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·W E ReiherT K Brunck
Aug 1, 1987·Journal of Clinical Microbiology·R S SmithD D Richman
Oct 1, 1973·European Journal of Immunology·M H JuliusL A Herzenberg

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Citations

Nov 12, 2015·The Journal of Biological Chemistry·Robyn Marty-RoixEgil Lien

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