PMID: 2500978Jul 11, 1989Paper

Synthetic peptides reproducing the EGF-receptor segment homologous to the pp60v-src phosphoacceptor site. Phosphorylation by tyrosine protein kinases

Biochimica Et Biophysica Acta
C ColaL A Pinna

Abstract

The octapeptide E-E-K-E-Y-H-A-E, corresponding to the amino acid sequence 841-845 of EGF receptor, whose tyrosine-845 is homologous to the main phosphorylation site of pp60v-src, has been synthesized together with seven shorter peptides encompassing variable segments around the tyrosine residue. The peptides have been employed as model substrates for inspecting the local structural determinants of three tyrosine protein kinases (TPKs), namely; TPK-IIB and TPK-III, isolated from lymphoid cells (Eur. J. Biochem. 172, 451-457 (1988] and the TPK encoded by the oncogene of Abelson murine leukemia virus. The phosphorylation order with the different peptide substrates is variable depending on the TPK used: in particular, the lysine residue at position -2 relative to tyrosine proved especially harmful with TPK-IIB, the peptides K-E-Y-H and K-E-Y-H-A-E being very poor substrates compared with their shorter derivatives devoid of the N-terminal lysine (E-Y-H and E-Y-H-A-E, respectively). Conversely, such a basic residue is well tolerated by the other two TPKs. The negative effect of the N-terminal lysine on TPK-IIB-catalyzed phosphorylation is accounted for by an increase of Km and can be overcome by the presence of additional glutamic ac...Continue Reading

References

Sep 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·B E KempE G Krebs
Jan 1, 1985·Annual Review of Biochemistry·T Hunter, J A Cooper
Mar 1, 1988·European Journal of Biochemistry·A M Brunati, L A Pinna
Oct 4, 1984·Nature·J DownwardM D Waterfield
Oct 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·J E SmartJ M Bishop

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Citations

Oct 1, 1994·The International Journal of Biochemistry·J A Boutin
Jun 2, 1989·Journal of Immunological Methods·S B KannerJ T Parsons
May 1, 1994·Free Radical Biology & Medicine·F UrsiniG Pifferi
Dec 27, 1990·European Journal of Biochemistry·A Donella-DeanaL A Pinna
Oct 15, 1991·European Journal of Biochemistry·A Donella-DeanaL A Pinna
Sep 1, 1994·European Journal of Biochemistry·J VeselýS Leclerc
Sep 1, 1989·Journal of Neuroscience Research·J M Pyper, J B Bolen
Aug 14, 1992·Biochemical and Biophysical Research Communications·F A GrässerN Mueller-Lantzsch
May 25, 2013·International Journal of Molecular Sciences·Ken-Ichi Sato
Dec 1, 1993·Journal of Biomolecular Structure & Dynamics·V S AnanthanarayananA D Bain
Mar 1, 1993·International Journal of Peptide and Protein Research·P RuzzaG Borin
Sep 20, 1996·Journal of Chromatography. B, Biomedical Applications·J A Boutin
May 26, 1995·Biochemical Pharmacology·P AgostinisP De Witte

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