PMID: 8973660Dec 1, 1996Paper

Synthetic phospho-oligosaccharide fragments of lipophosphoglycan as acceptors for Leishmania major alpha-D-mannosylphosphate transferase

European Journal of Biochemistry
G M BrownM A Ferguson

Abstract

Protozoan parasites of the genus Leishmania synthesise lipophosphoglycans, phosphoglycans and proteophosphoglycans that contain phosphosaccharide-repeat units of [-6Gal beta 1-4Man alpha 1-P-]. In this study, a GDP-Man-dependent alpha-mannosylphosphate-transferase activity was detected in washed Leishmania major membranes using synthetic phospho-oligosaccharide fragments of lipophosphoglycan as acceptor substrates. The divalent-cation-dependent alpha-mannosylphosphate-transferase activity had an apparent K(m) for GDP-Man of about 15-20 microM and a pH optimum of 7.0. The activity showed a requirement for a non-reducing terminal beta Gal residue and for one or more phosphodiester units preceding the acceptor site. Based on these results, the activity may be defined as a GDP-Man: Gal beta 1-4Man alpha 1-P-R alpha-mannosylphosphate-transferase. This acceptor specificity is consistent with a role for the alpha-mannosylphosphate transferase in the elongation of phosphosaccharide-repeat domains of Leishmania glycoconjugates rather than in the priming of these domains. An identical or similar activity must exist in the amastigote forms of the Leishmania that produce and secrete proteophosphoglycan material and the activity therefore r...Continue Reading

References

Sep 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·K A RyanS M Beverley

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Citations

Nov 7, 2000·Parasitology Today·T Ilg
Nov 18, 1998·Current Opinion in Structural Biology·B J Mengeling, S J Turco
May 17, 2001·Bioorganic & Medicinal Chemistry·J R BrownD Chatterjec
Jul 20, 2010·Biochemistry. Biokhimii︠a︡·N M Novozhilova, N V Bovin
Jun 29, 1999·Biological Chemistry·A TiedeR E Schmidt
Feb 26, 1999·The Journal of Biological Chemistry·J M MossM J McConville
Jun 23, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Mukesh SamantAnuradha Dube
Jun 9, 2001·The Journal of Organic Chemistry·M C Hewitt, P H Seeberger

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