Synthetic progestins medroxyprogesterone acetate and dydrogesterone and their binary mixtures adversely affect reproduction and lead to histological and transcriptional alterations in zebrafish (Danio rerio)

Environmental Science & Technology
Yanbin ZhaoKarl Fent

Abstract

Medroxyprogesterone acetate (MPA) and dydrogesterone (DDG) are synthetic progestins widely used in human and veterinary medicine. Although aquatic organisms are exposed to them through wastewater and animal farm runoff, very little is known about their effects in the environment. Here we provide a comprehensive analysis of the responses of zebrafish (Danio rerio) to MPA, DDG, and their binary mixtures at measured concentrations between 4.5 and 1663 ng/L. DDG and both mixtures impaired reproductive capacities (egg production) of breeding pairs and led to histological alterations of ovaries and testes and increased gonadosomatic index. Transcriptional analysis of up to 28 genes belonging to different pathways demonstrated alterations in steroid hormone receptors, steroidogenesis enzymes, and specifically, the circadian rhythm genes, in different organs of adult zebrafish and eleuthero-embryos. Alterations occurred even at environmentally relevant concentrations of 4.5-4.8 ng/L MPA, DDG and the mixture in eleuthero-embryos and at 43-89 ng/L in adult zebrafish. Additionally, the mixtures displayed additive effects in most but not all parameters in adults and eleuthero-embryos, suggesting concentration addition. Our data suggest tha...Continue Reading

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Citations

Jun 2, 2016·Toxicology and Applied Pharmacology·Joel Cano-NicolauFrançois Brion
Jun 25, 2016·Journal of Molecular Endocrinology·Silvia GiattiMarzia Pesaresi
Sep 13, 2016·Environmental Pollution·Fangjie CaoLihong Qiu
Feb 12, 2021·Bulletin of Environmental Contamination and Toxicology·Wen-Jun ShiGuang-Guo Ying
Nov 10, 2020·General and Comparative Endocrinology·Lisa N Bottalico, Aalim M Weljie
Jul 14, 2018·Environmental Science & Technology·Wen-Jun ShiGuang-Guo Ying
Jul 12, 2020·Environmental Science & Technology·Clémentine GarocheFrançois Brion
Mar 28, 2018·Environmental Science & Technology·Alexander WeizelThomas A Ternes
Mar 3, 2020·Ecotoxicology and Environmental Safety·Wen-Jun ShiGuang-Guo Ying

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