Synthetic recombinant bat SARS-like coronavirus is infectious in cultured cells and in mice.

Proceedings of the National Academy of Sciences of the United States of America
Michelle M BeckerMark R Denison


Defining prospective pathways by which zoonoses evolve and emerge as human pathogens is critical for anticipating and controlling both natural and deliberate pandemics. However, predicting tenable pathways of animal-to-human movement has been hindered by challenges in identifying reservoir species, cultivating zoonotic organisms in culture, and isolating full-length genomes for cloning and genetic studies. The ability to design and recover pathogens reconstituted from synthesized cDNAs has the potential to overcome these obstacles by allowing studies of replication and pathogenesis without identification of reservoir species or cultivation of primary isolates. Here, we report the design, synthesis, and recovery of the largest synthetic replicating life form, a 29.7-kb bat severe acute respiratory syndrome (SARS)-like coronavirus (Bat-SCoV), a likely progenitor to the SARS-CoV epidemic. To test a possible route of emergence from the noncultivable Bat-SCoV to human SARS-CoV, we designed a consensus Bat-SCoV genome and replaced the Bat-SCoV Spike receptor-binding domain (RBD) with the SARS-CoV RBD (Bat-SRBD). Bat-SRBD was infectious in cell culture and in mice and was efficiently neutralized by antibodies specific for both bat and...Continue Reading


Dec 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·M A HofmannD A Brian
Apr 1, 1997·Virus Research·R Buckland, T F Wild
Jan 10, 2003·Nucleic Acids Research·Kim D PruittDonna R Maglott
Mar 29, 2003·Journal of Virology·Bert Jan HaijemaPeter J M Rottier
Apr 12, 2003·The New England Journal of Medicine·Christian DrostenHans Wilhelm Doerr
Apr 12, 2003·The New England Journal of Medicine·Thomas G KsiazekUNKNOWN SARS Working Group
Apr 25, 2003·Lancet·J S M PeirisUNKNOWN SARS study group
Oct 22, 2003·Proceedings of the National Academy of Sciences of the United States of America·Boyd YountRalph S Baric
Dec 6, 2003·Proceedings of the National Academy of Sciences of the United States of America·Hamilton O SmithJ Craig Venter
Dec 13, 2003·The Journal of Biological Chemistry·Swee Kee WongMichael Farzan
Dec 13, 2003·Journal of Virology·John Stavrinides, David S Guttman
Aug 13, 2004·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Edward C Holmes, Andrew Rambaut
Dec 4, 2004·Nature Medicine·Robin A Weiss, Anthony J McMichael
Dec 4, 2004·Nature Medicine·Richard WebbyRobert Webster
Jan 25, 2005·Emerging Infectious Diseases·Changchun TuLin-Fa Wang
Mar 26, 2005·The EMBO Journal·Wenhui LiMichael Farzan
Aug 27, 2005·Virology Journal·Samitabh ChakrabortiDimiter S Dimitrov
Sep 20, 2005·Proceedings of the National Academy of Sciences of the United States of America·Susanna K P LauKwok-Yung Yuen
Oct 1, 2005·Science·Wendong LiLin-Fa Wang
Oct 8, 2005·Science·Terrence M TumpeyAdolfo García-Sastre
Feb 21, 2006·Emerging Infectious Diseases·Mark E J Woolhouse, Sonya Gowtage-Sequeria
Jun 9, 2006·Trends in Microbiology·Kanta Subbarao, Anjeanette Roberts
Jul 19, 2006·Clinical Microbiology Reviews·Charles H CalisherTony Schountz
Jan 16, 2007·PLoS Pathogens·Anjeanette RobertsKanta Subbarao
Jan 30, 2007·PLoS Pathogens·Young Nam Lee, Paul D Bieniasz
Feb 6, 2007·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Kim HalpinUNKNOWN Henipavirus Ecology Research Group
Apr 25, 2007·Virus Research·Zhengli Shi, Zhihong Hu
May 15, 2007·Virus Research·Anjeanette RobertsKanta Subbarao
Sep 18, 2007·Current Opinion in Structural Biology·Robert A Lamb, Theodore S Jardetzky

❮ Previous
Next ❯


Nov 5, 2013·Antiviral Research·Jan Felix DrexlerChristian Drosten
Mar 15, 2013·Nature·Tom Gallagher, Stanley Perlman
Dec 17, 2009·Nature Biotechnology·Eckard WimmerJeffery K Taubenberger
Apr 17, 2010·Nature Reviews. Genetics·Ahmad S Khalil, James J Collins
Nov 30, 2011·Nature Reviews. Genetics·Wilfried Weber, Martin Fussenegger
May 12, 2009·Nature Reviews. Microbiology·Stanley Perlman, Jason Netland
Sep 18, 2013·Proceedings of the National Academy of Sciences of the United States of America·Trevor ScobeyRalph S Baric
Sep 21, 2012·Journal of Virology·Jeremy HuynhEric F Donaldson
May 2, 2014·Nature Methods·Sriram Kosuri, George M Church
Jun 3, 2014·Genome Biology·Bret Cooper
Jun 17, 2014·Virus Research·Fernando AlmazánLuis Enjuanes
Jul 16, 2011·Annual Review of Microbiology·Eckard Wimmer, Aniko V Paul
Jun 27, 2014·Viruses·Christopher C Stobart, Martin L Moore
May 26, 2012·Metabolic Engineering·Chuck Merryman, Daniel G Gibson
Dec 20, 2011·Current Opinion in Virology·Meagan BollesRalph Baric
Aug 31, 2010·International Journal of Antimicrobial Agents·Larry J Anderson, Suxiang Tong
Jul 8, 2009·Drug Discovery Today·Wilfried Weber, Martin Fussenegger
Nov 10, 2015·Nature Medicine·Vineet D MenacheryRalph S Baric
Jul 15, 2009·Biochemical and Biophysical Research Communications·Peng ZhouZhengli Shi
Oct 4, 2014·Virus Research·Vineet D MenacheryRalph S Baric
Mar 20, 2012·Human Vaccines & Immunotherapeutics·Chen ZhaoEnjie Luo

❮ Previous
Next ❯

Related Concepts

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.


Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.


Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.