Synthetic recombinant bat SARS-like coronavirus is infectious in cultured cells and in mice.

Proceedings of the National Academy of Sciences of the United States of America
Michelle M BeckerMark R Denison

Abstract

Defining prospective pathways by which zoonoses evolve and emerge as human pathogens is critical for anticipating and controlling both natural and deliberate pandemics. However, predicting tenable pathways of animal-to-human movement has been hindered by challenges in identifying reservoir species, cultivating zoonotic organisms in culture, and isolating full-length genomes for cloning and genetic studies. The ability to design and recover pathogens reconstituted from synthesized cDNAs has the potential to overcome these obstacles by allowing studies of replication and pathogenesis without identification of reservoir species or cultivation of primary isolates. Here, we report the design, synthesis, and recovery of the largest synthetic replicating life form, a 29.7-kb bat severe acute respiratory syndrome (SARS)-like coronavirus (Bat-SCoV), a likely progenitor to the SARS-CoV epidemic. To test a possible route of emergence from the noncultivable Bat-SCoV to human SARS-CoV, we designed a consensus Bat-SCoV genome and replaced the Bat-SCoV Spike receptor-binding domain (RBD) with the SARS-CoV RBD (Bat-SRBD). Bat-SRBD was infectious in cell culture and in mice and was efficiently neutralized by antibodies specific for both bat and...Continue Reading

References

Dec 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·M A HofmannD A Brian
Apr 1, 1997·Virus Research·R Buckland, T F Wild
Jan 10, 2003·Nucleic Acids Research·Kim D PruittDonna R Maglott
Mar 29, 2003·Journal of Virology·Bert Jan HaijemaPeter J M Rottier
Apr 12, 2003·The New England Journal of Medicine·Christian DrostenHans Wilhelm Doerr
Apr 12, 2003·The New England Journal of Medicine·Thomas G KsiazekUNKNOWN SARS Working Group
Apr 25, 2003·Lancet·J S M PeirisUNKNOWN SARS study group
Oct 22, 2003·Proceedings of the National Academy of Sciences of the United States of America·Boyd YountRalph S Baric
Dec 6, 2003·Proceedings of the National Academy of Sciences of the United States of America·Hamilton O SmithJ Craig Venter
Dec 13, 2003·The Journal of Biological Chemistry·Swee Kee WongMichael Farzan
Dec 13, 2003·Journal of Virology·John Stavrinides, David S Guttman
Aug 13, 2004·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Edward C Holmes, Andrew Rambaut
Dec 4, 2004·Nature Medicine·Robin A Weiss, Anthony J McMichael
Dec 4, 2004·Nature Medicine·Richard WebbyRobert Webster
Jan 25, 2005·Emerging Infectious Diseases·Changchun TuLin-Fa Wang
Mar 26, 2005·The EMBO Journal·Wenhui LiMichael Farzan
Aug 27, 2005·Virology Journal·Samitabh ChakrabortiDimiter S Dimitrov
Sep 20, 2005·Proceedings of the National Academy of Sciences of the United States of America·Susanna K P LauKwok-Yung Yuen
Oct 1, 2005·Science·Wendong LiLin-Fa Wang
Oct 8, 2005·Science·Terrence M TumpeyAdolfo García-Sastre
Feb 21, 2006·Emerging Infectious Diseases·Mark E J Woolhouse, Sonya Gowtage-Sequeria
Jun 9, 2006·Trends in Microbiology·Kanta Subbarao, Anjeanette Roberts
Jul 19, 2006·Clinical Microbiology Reviews·Charles H CalisherTony Schountz
Jan 16, 2007·PLoS Pathogens·Anjeanette RobertsKanta Subbarao
Jan 30, 2007·PLoS Pathogens·Young Nam Lee, Paul D Bieniasz
Feb 6, 2007·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Kim HalpinUNKNOWN Henipavirus Ecology Research Group
Apr 25, 2007·Virus Research·Zhengli Shi, Zhihong Hu
May 15, 2007·Virus Research·Anjeanette RobertsKanta Subbarao
Sep 18, 2007·Current Opinion in Structural Biology·Robert A Lamb, Theodore S Jardetzky

❮ Previous
Next ❯

Citations

Nov 5, 2013·Antiviral Research·Jan Felix DrexlerChristian Drosten
Mar 15, 2013·Nature·Tom Gallagher, Stanley Perlman
Dec 17, 2009·Nature Biotechnology·Eckard WimmerJeffery K Taubenberger
Apr 17, 2010·Nature Reviews. Genetics·Ahmad S Khalil, James J Collins
Nov 30, 2011·Nature Reviews. Genetics·Wilfried Weber, Martin Fussenegger
May 12, 2009·Nature Reviews. Microbiology·Stanley Perlman, Jason Netland
Sep 18, 2013·Proceedings of the National Academy of Sciences of the United States of America·Trevor ScobeyRalph S Baric
Sep 21, 2012·Journal of Virology·Jeremy HuynhEric F Donaldson
May 2, 2014·Nature Methods·Sriram Kosuri, George M Church
Jun 3, 2014·Genome Biology·Bret Cooper
Jun 17, 2014·Virus Research·Fernando AlmazánLuis Enjuanes
Jul 16, 2011·Annual Review of Microbiology·Eckard Wimmer, Aniko V Paul
Jun 27, 2014·Viruses·Christopher C Stobart, Martin L Moore
May 26, 2012·Metabolic Engineering·Chuck Merryman, Daniel G Gibson
Dec 20, 2011·Current Opinion in Virology·Meagan BollesRalph Baric
Aug 31, 2010·International Journal of Antimicrobial Agents·Larry J Anderson, Suxiang Tong
Jul 8, 2009·Drug Discovery Today·Wilfried Weber, Martin Fussenegger
Nov 10, 2015·Nature Medicine·Vineet D MenacheryRalph S Baric
Jul 15, 2009·Biochemical and Biophysical Research Communications·Peng ZhouZhengli Shi
Oct 4, 2014·Virus Research·Vineet D MenacheryRalph S Baric
Mar 20, 2012·Human Vaccines & Immunotherapeutics·Chen ZhaoEnjie Luo

❮ Previous
Next ❯

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