Synthetic resin-bound truncated Candida antarctica lipase B for production of fatty acid alkyl esters by transesterification of corn and soybean oils with ethanol or butanol

Journal of Biotechnology
Stephen R HughesPaolo Caimi

Abstract

A gene encoding a synthetic truncated Candida antarctica lipase B (CALB) was generated via automated PCR and expressed in Saccharomyces cerevisiae. Western blot analysis detected five truncated CALB variants, suggesting multiple translation starts from the six in-frame ATG codons. The longest open reading frame, which corresponds to amino acids 35-317 of the mature lipase, appeared to be expressed in the greatest amount. The truncated CALB was immobilized on Sepabeads® EC-EP resin and used to produce ethyl and butyl esters from crude corn oil and refined soybean oil. The yield of ethyl esters was 4-fold greater from corn oil than from soybean oil and was 36% and 50% higher, respectively, when compared to a commercially available lipase resin (Novozym 435) using the same substrates. A 5:1 (v/v) ratio of ethanol to corn oil produced 3.7-fold and 8.4-fold greater yields than ratios of 15:1 and 30:1, respectively. With corn oil, butyl ester production was 56% higher than ethyl ester production. Addition of an ionic catalytic resin step prior to the CALB resin increased yields of ethyl esters from corn oil by 53% compared to CALB resin followed by ionic resin. The results suggest resin-bound truncated CALB has potential application ...Continue Reading

References

Aug 28, 1998·Chemistry and Physics of Lipids·S PatkarO Kirk
Nov 1, 2001·Proceedings of the National Academy of Sciences of the United States of America·S R ThompsonP Sarnow
Jan 1, 1996·Biotechnology Advances·Z ZhangY Chisti
Oct 11, 2003·Biotechnology Advances·R SharmaU C Banerjee
May 11, 2005·Chembiochem : a European Journal of Chemical Biology·Anders O MagnussonKarl Hult
Oct 20, 2005·Journal of Bioscience and Bioengineering·H FukudaH Noda
Jun 27, 2007·Bioresource Technology·Srivathsan Vembanur RanganathanKaruppan Muthukumar
Oct 2, 2007·Journal of Agricultural and Food Chemistry·Casimir C AkohJei-Fu Shaw
Jan 22, 2008·Journal of Industrial Microbiology & Biotechnology·Palligarnai T Vasudevan, Michael Briggs
May 15, 2008·Applied Biochemistry and Biotechnology·Otávio L BernardesMarta A P Langone
Feb 14, 2009·Biotechnology and Bioengineering·Lene FjerbaekBirgir Norddahl
Jun 29, 2010·Biotechnology Advances·Tianwei TanFang Wang

❮ Previous
Next ❯

Citations

Sep 5, 2015·International Journal of Molecular Sciences·Grazia M Borrelli, Daniela Trono
Jun 12, 2013·Marine Biotechnology·Chongfu SongXiaobo Zhang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Biofuels (ASM)

Biofuels are produced through contemporary processes from biomass rather than geological processes involved in fossil fuel formation. Examples include biodiesel, green diesel, biogas, etc. Discover the latest research on biofuels in this feed.