Synthetic RNA technologies to control functions of mammalian cells

Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan
Hirohide Saito

Abstract

We recently succeeded in producing nanostructures made of RNA-protein (RNP) complexes. We show that RNA and the ribosomal protein L7Ae can form a triangular-like nanostructure that consists of three L7Ae proteins, which form the apices of the triangle, bound to one RNA scaffold. This shape is created through a 60° kink introduced into the RNA structure on L7Ae binding. By varying the size of the RNA scaffold we could in turn alter the overall size of the triangular nanostructure. Several functions can be added to this nanostructure by the introduction of effector proteins fused to L7Ae. The design and construction of functional RNP nanostructures that detect specific cancer cells are discussed herein. In parallel, we developed synthetic RNP translational switches to control production levels of particular proteins depending on certain input(s) within the intracellular environment. The RNP-binding module was successfully incorporated into mRNA to generate functional RNP switches. The designed ON/OFF translational switches detect expression of the trigger factor and repress or activate expression of a desired protein (e.g., apoptosis regulator) in target mammalian cells. Taken together, RNP-binding module could be employed for co...Continue Reading

References

Apr 4, 2001·The EMBO Journal·H SaitoH Suga
Aug 3, 2001·The EMBO Journal·D J KleinT A Steitz
Aug 20, 2002·Proceedings of the National Academy of Sciences of the United States of America·Jeffrey L HansenThomas A Steitz
Dec 18, 2004·Science·Arkadiusz ChworosLuc Jaeger
Mar 9, 2006·Nucleic Acids Research·Lorena NasaleanLuc Jaeger
Dec 18, 2009·Nature Chemical Biology·Hirohide SaitoTan Inoue
Jan 18, 2011·Nature Nanotechnology·Hirohisa OhnoHirohide Saito
Jan 20, 2011·Nature Communications·Hirohide SaitoTan Inoue
Feb 22, 2012·Science·Shawn M DouglasGeorge M Church
Jul 20, 2012·Nucleic Acids Research·Shunnichi KashidaHirohide Saito
Feb 6, 2013·Chemical Communications : Chem Comm·Tomoaki HaraTan Inoue
May 21, 2013·Nucleic Acids Research·Kei EndoTan Inoue
Sep 4, 2013·Nature Communications·Kei EndoHirohide Saito
Jun 7, 2014·Accounts of Chemical Research·Masami HagiyaSatoshi Murata

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.