Synthetic serum substitute (SSS): a globulin-enriched protein supplement for human embryo culture

Journal of Assisted Reproduction and Genetics
P S WeathersbeeT Ord

Abstract

The purpose of the present study was to evaluate whether an IVF protein supplement prepared from human serum albumin (HSA) and human globulins would retain performance characteristics equivalent to those reported for the commercial plasma expanders, Plasmatein (Alpha Therapeutics, Los Angeles, California) and Plasmanate (Cutter Biological, Miles Inc., Elkhart, Indiana). Pronuclear-stage human embryos were randomly divided and cultured in human tubal fluid medium (HTF) supplemented with either HSA (5 mg/mL) or Plasmatein (10%, v/v; 5 mg/ml) as a means of indirectly assessing the effect alpha- and beta-globulins have on embryonic development. Those results coupled with the known composition characteristics of Plasmatein were used as the starting basis to formulate test lots of synthetic serum substitute (SSS). Significantly (P < 0.05) more of the human embryos cultured in Plasmatein supplemented medium reached the four-cell or greater stage by 40 hr postinsemination than a comparable group cultured in HSA alone. Lot 1 of SSS, formulated with HSA (84% of total protein) and human globulins (16% of total protein) and an aqueous lipoprotein fraction derived from human plasma (Excyte IV; Miles Diagnostics, Kankakee, Illinois), produce...Continue Reading

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Citations

May 1, 1996·Journal of Assisted Reproduction and Genetics·J FrasorR G Rawlins
Oct 1, 1996·Obstetrics and Gynecology·K F MillerT Falcone
Jun 1, 2001·Fertility and Sterility·N A CekleniakC Racowsky
Jun 10, 2004·Journal of Assisted Reproduction and Genetics·Jennifer E GravesWilliam R Boone
Dec 1, 2004·Journal of Assisted Reproduction and Genetics·Dalit Ben-YosefYuval Yaron
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Sep 28, 2014·Journal of Assisted Reproduction and Genetics·Dean E MorbeckDietrich Matern
Feb 15, 2002·Human Fertility : Journal of the British Fertility Society·Thomas G. McEvoyJohn J. Robinson
Sep 8, 2015·Journal of Assisted Reproduction and Genetics·Jolene FredricksonDean E Morbeck
Mar 17, 2017·Systems Biology in Reproductive Medicine·Alessandra A VirequeAna Carolina J Sá Rosa-E-Silva
Apr 18, 2018·Current Opinion in Obstetrics & Gynecology·Diego Marin, Richard T Scott
Dec 31, 2014·Endocrine Reviews·George A ThouasDavid K Gardner
Feb 28, 2019·Stem Cell Research & Therapy·Wojciech ZakrzewskiZbigniew Rybak
Jun 15, 2017·Journal of Developmental Origins of Health and Disease·D K Gardner, R L Kelley
Jan 15, 2021·Zygote : the Biology of Gametes and Early Embryos·Sam Zarbakhsh

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