Synthetic strategies and parameters involved in the synthesis of oligodeoxyribonucleotides according to the phosphoramidite method

Current Protocols in Nucleic Acid Chemistry
S L Beaucage, M H Caruthers

Abstract

The phosphoramidite approach has had a major impact on the synthesis of oligonucleotides. This unit describes parameters that affect the performance of this method for preparing oligodeoxyribonucleotides, as well as a number of compatible strategies. Milestones that led to the discovery of the approach are chronologically reported. Alternate strategies are also described to underscore the versatility by which these synthons can be obtained. Mechanisms of deoxyribonucleoside phosphoramidite activation, factors affecting condensation, and deprotection strategies are discussed.

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Citations

Dec 1, 2017·American Journal of Hypertension·Roopesh S GangwarJeffrey A Deiuliis
Aug 3, 2017·International Journal of Molecular Sciences·Shuaijian NiGe Zhang
Jan 31, 2006·Journal of the American Society for Mass Spectrometry·Keiichiro OharaJean-Jacques Vasseur
Sep 2, 2016·Current Protocols in Nucleic Acid Chemistry·Magdalena Petrová, Ivan Rosenberg
Mar 12, 2016·Current Protocols in Nucleic Acid Chemistry·Rémy Lartia
Feb 12, 2008·Current Protocols in Molecular Biology·J PollardA D Ellington
Apr 21, 2020·Current Protocols in Nucleic Acid Chemistry·Ashok Nuthanakanti, Seergazhi G Srivatsan
Jul 7, 2020·Current Protocols in Nucleic Acid Chemistry·Shiyue FangBhaskar Halami
Mar 1, 2016·Current Protocols in Nucleic Acid Chemistry·Ivo Sarac, Chris Meier
Oct 10, 2009·Current Protocols in Molecular Biology·Bradley HallAndrew D Ellington
Sep 8, 2015·Current Protocols in Nucleic Acid Chemistry·Li WuXinjing Tang

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