SypHer3s: a genetically encoded fluorescent ratiometric probe with enhanced brightness and an improved dynamic range

Chemical Communications : Chem Comm
Yulia G ErmakovaVsevolod V Belousov

Abstract

We designed a genetically encoded ratiometric fluorescent probe, SypHer3s, with enhanced brightness and optimized pKa, which responds to pH changes in different cellular compartments. SypHer3s was successfully utilized for imaging the pH dynamics in mitochondria of living neurons and in quantitative pH measurement in zebrafish embryos.

References

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Citations

Aug 30, 2019·International Journal of Molecular Sciences·Alexander I KostyukDmitry S Bilan
Jun 18, 2020·Antioxidants·Alexander I KostyukDmitry S Bilan
Jun 15, 2019·Optics Letters·M S PochechuevA M Zheltikov
Nov 19, 2020·Journal of Biophotonics·Artem S ChebotarevAleksei M Zheltikov
Dec 31, 2020·International Journal of Molecular Sciences·Ekaterina S PotekhinaDmitry S Bilan
Jul 15, 2020·Trends in Biochemical Sciences·Xin ZhouJin Zhang
Jul 31, 2021·Optics Letters·M S PochechuevA M Zheltikov
Jun 27, 2021·The FEBS Journal·Daria D SmolyarovaVsevolod V Belousov

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Methods Mentioned

BETA
Fluorescence
gel-filtration
transfection
biosensor

Software Mentioned

SypHer3s
HyPer
SypHer

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