Syringe needle-based sampling coupled with liquid-phase extraction for determination of the three-dimensional distribution of l-ascorbic acid in apples

Food Chemistry
Sheng Tang, Hian Kee Lee

Abstract

A novel syringe needle-based sampling approach coupled with liquid-phase extraction (NBS-LPE) was developed and applied to the extraction of l-ascorbic acid (AsA) in apple. In NBS-LPE, only a small amount of apple flesh (ca. 10mg) was sampled directly using a syringe needle and placed in a glass insert for liquid extraction of AsA by 80μL oxalic acid-acetic acid. The extract was then directly analyzed by liquid chromatography. This new procedure is simple, convenient, almost organic solvent free, and causes far less damage to the fruit. To demonstrate the applicability of NBS-LPE, AsA levels at different sampling points in a single apple were determined to reveal the spatial distribution of the analyte in a three-dimensional model. The results also showed that this method had good sensitivity (limit of detection of 0.0097mg/100g; limit of quantification of 0.0323mg/100g), acceptable reproducibility (relative standard deviation of 5.01% (n=6)), a wide linear range of between 0.05 and 50mg/100g, and good linearity (r(2)=0.9921). This interesting extraction technique and modeling approach can be used to measure and monitor a wide range of compounds in various parts of different soft-matrix fruits and vegetables, including single s...Continue Reading

References

Jul 18, 2006·Plant Physiology·Mark W DaveyJohan Keulemans
Nov 26, 2009·Journal of Separation Science·Yingjia YuGengli Duan
Jul 4, 2012·Journal of the Science of Food and Agriculture·Audrey PissardMarc Lateur
Dec 12, 2012·Journal of Separation Science·Lukman Bola Abdulra'ufGuan Huat Tan

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